+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

T antigen repression of SV40 early transcription from two promoters



T antigen repression of SV40 early transcription from two promoters



Cell 27(3 Pt 2): 603-613



The SV40 early mRNAs encode large (T) and small (t) tumor antigens. During the lytic cycle, the 5' termini of the early mRNAs undergo a shift: shortly after infection only an initiation site downstream from the TATA box is utilized; later an upstream initiation site becomes prominent. Both initiation sites are utilized in an in vitro transcription extract. D2T, a T antigen analog, specifically represses transcription in vitro from both initiation sites, but at different concentrations. Binding of D2T to site I suppresses initiation from the site downstream of the TATA box; binding to sites II and I suppresses initiation from the upstream site. The role of T antigen binding in repression of transcription and in the shift of initiation sites on the early strand is discussed.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 044512959

Download citation: RISBibTeXText

PMID: 6101224

DOI: 10.1016/0092-8674(81)90402-5


Related references

T antigen repression of sv 40 early transcription from 2 promoters. Cell 27(3 Part 2): 603-612, 1981

Transcription in vivo from SV40 early promoter deletion mutants without repression by large T antigen. Journal of Molecular and Applied Genetics 2(1): 127-135, 1983

Involvement of simian virus 40 (SV40) small t antigen in trans activation of SV40 early and late promoters. Journal of Virology 66(3): 1489-1494, 1992

Initiation of transcription by RNA polymerase II in permeable, SV40-infected or noninfected, CVI cells; evidence for multiple promoters of SV40 late transcription. Nucleic Acids Research 9(2): 215-236, 1981

Initiation of transcription by Rna polymerase Ii in permeable, Sv40-infected or noninfected, Cv1 cells; evidence for multiple promoters of Sv40 late transcription. Nucleic Acids Research 9(2): 215-236, 1981

Transcription from the SV40 early-early and late-early overlapping promoters in the absence of DNA replication. Embo Journal 2(9): 1605-1611, 1983

Transcription from the Sv40 early-early and late-early overlapping promoters in the absence of Dna replication. The Embo Journal 2(9): 1605-1611, 1983

A 47-amino-acid fragment of SV40 T antigen represses transcription from human GSTalpha promoters. Virology 249(2): 275-285, 1998

A 47-amino-acid fragment of SV40 T antigen represses transcription from human GSTa promoters. Virology 249(2): 5-85, 1998

pRb, Myc and p53 are critically involved in SV40 large T antigen repression of PDGF beta-receptor transcription. Journal of Cell Science 117(Pt 17): 3855-3865, 2004

Effect of the 21-bp repeat upstream element on in vitro transcription from the early and late Sv40 promoters. The Embo Journal 3(10): 2373-2382, 1984

Effect of the 21-bp repeat upstream element on in vitro transcription from the early and late SV40 promoters. Embo Journal 3(10): 2373-2382, 1984

Nucleosome positioning in the regulatory region of SV40 chromatin correlates with the activation and repression of early and late transcription during infection. Virology 503: 62-69, 2017

Repression of two proliferation proteins during senescence and terminal differentiation in normal or SV40 transfected human keratinocytes P2P2 and SV40 large T antigen. Molecular & Cellular Differentiation 1(2): 185-195, 1993

Role of HU and DNA supercoiling in transcription repression: Specialized nucleoprotein repression complex at gal promoters in Escherichia coli. Molecular Microbiology 31(2): 451-461, 1999