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The 'pricking' method. A new efficient technique for mechanically introducing foreign DNA into the nuclei of culture cells

, : The 'pricking' method. A new efficient technique for mechanically introducing foreign DNA into the nuclei of culture cells. Experimental Cell Research 142(1): 79-84

Using the thymidine kinase gene-containing plasmid pTK-1 and the mouse fibroblast thymidine kinase-deficient LTK- cell line, we obtained results indicating that the plasmid molecules suspended in the external medium were introduced into the nuclei by pricking cells on the nuclei with a microneedle. Approx. 25% of the cells pricked in the presence of the plasmid pTK-1 showed TK gene expression, and 2% of the cells became stable TK+ transformants. This 'pricking' method is applicable for introducing DNA into cell nuclei and is more efficient than the conventional calcium phosphate transformation method.

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Accession: 044534929

PMID: 6958471

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Other references

Fridland V. M., 1975: The concept of facies in soil science. Izvestiya Akademii Nauk SSSR Seriya Geograficheskaya

Arad, Zeev., 1993: Effect of desiccation on the water economy of terrestrial gastropods of different phylogenetic origins: a prosobranch (Pomatias glaucus) and two pulmonates (Sphincterochila cariosa and Helix engaddensis). This study compares the water relations and resistance to desiccation in three species of land snails of different phylogenetic origins: the prosobranch Pomatias glaucus and the pulmonates Helix engaddensis and Sphincterochila cariosa. All three s...

Pedrosa, C.A.; Ahern, D.K.; McKenna, M.J.; Ojemann, R.G.; Acquadro, M.A., 1994: Determinants and impact of headache after acoustic neuroma surgery. Headache after acoustic neuroma surgery is known to occur clinically, but has not been studied systematically until recently. In the present study, 155 patients were surveyed regarding their experience of headache and associated symptoms following...

Shi, John Xi; Le Maguer, Marc; Wang, Samuel L.; Liptay, Albert, 1997: Application of osmotic treatment in tomato processing-effect of skin treatments on mass transfer in osmotic dehydration of tomatoes. Osmotic dehydration can be a method to minimize negative modifications of fresh food components during processing. Cut tomatoes are poorly suited to osmotic dehydration because of their heterogeneous texture and potential excessive loss of liquid...

Sone S.; Ishii K.; Tsuruo T., 1986: Enhanced cytosine arabinoside resistance in human kb epidermoid carcinoma cells on induction of a second resistance to vincristine. Uicc (Union Internationale Contre Le Cancer, International Union Against Cancer) 14th International Cancer Congress, Budapest, Hungary, Aug 21-27, Abstracts, Lectures, Symposia And Free Communications, Vols 1, 2, 3, Late Abstracts, And Register Xvi+479p (Vol 1); Xvi+298p (Vol 2); Xvi+531p (Vol 3); 15p (Late Abstracts); 40p (Register) S Karger Ag: Basel, Switzerland; New York, N Y , Usa; Akademiai Kiado: Budapest, Hungary Paper 167

Oldham, R.K.; Greco, F.A., 1980: Small-cell lung cancer. A curable disease. Cancer ChemoTherapy and Pharmacology 4(3): 173-177

Lemberg, K.K.; Kontinen, V.K.; Siiskonen, A.O.; Viljakka, K.M.; Yli-Kauhaluoma, J.T.; Korpi, E.R.; Kalso, E.A., 2006: Antinociception by spinal and systemic oxycodone: why does the route make a difference? In vitro and in vivo studies in rats. Background: The pharmacology of oxycodone is poorly understood despite its growing clinical use. The discrepancy between its good clinical effectiveness after systemic administration and the loss of potency after spinal administration led the auth...

Aleksandrov, N.N.; Sukovatykh, L.S.; Fradkin, S.Z.; Shuster, L.A., 1968: On ways of interpreting"postoperative complications"and"postoperative mortality"in tumor patients (published in order of controversy). Voprosy Onkologii 14(11): 39-43

S. V. Kukhtii, 1976: Further improvements to engineering-geologic investigations for hydrotechnical construction. Power Technology and Engineering 10(12): 1235-1237

Korsmeyer R.W.; Peppas N.A., 1981: Effect of the morphology of hydrophilic polymeric matrices on the diffusion and release of water soluble drugs. Drug release mechanism from, and diffusion processes in, hydrophilic cross-linked polymeric systems were investigated in 2 macromolecular states: in the glassy and rubbery states during the early part of countercurrent water diffusion, and in the...