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The 'pricking' method. A new efficient technique for mechanically introducing foreign DNA into the nuclei of culture cells


, : The 'pricking' method. A new efficient technique for mechanically introducing foreign DNA into the nuclei of culture cells. Experimental Cell Research 142(1): 79-84

Using the thymidine kinase gene-containing plasmid pTK-1 and the mouse fibroblast thymidine kinase-deficient LTK- cell line, we obtained results indicating that the plasmid molecules suspended in the external medium were introduced into the nuclei by pricking cells on the nuclei with a microneedle. Approx. 25% of the cells pricked in the presence of the plasmid pTK-1 showed TK gene expression, and 2% of the cells became stable TK+ transformants. This 'pricking' method is applicable for introducing DNA into cell nuclei and is more efficient than the conventional calcium phosphate transformation method.

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Accession: 044534929

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