The acid-soluble fraction of amyloid--a fibril forming protein
Pras, M.; Reshef, T.
Biochimica et Biophysica Acta 271(1): 193-203
ISSN/ISBN: 0006-3002 PMID: 4261030 DOI: 10.1016/0005-2795(72)90147-x
Warming of five amyloid suspensions in 0.15 M Na Cl made in 0.02 M HCl at 60 °C for 1 h, resulted in solubilization of 50% of their dry weight. The acid-soluble fraction of the amyloid fiber was soluble in 0.007 M to 0.08 M HCl, contained 16% N and did not contain measurable amounts of mucopolysaccharides and free sugars. Amino acid analyses showed a predominance of alanine and aspartic acid and a lack of cysteine and methionine. The sedimentation coefficient of the acid-soluble fraction was found to be between 0.64 and 0.88 S; its molecular weight by sodium dodecyl sulfate polyacrylamide electrophoresis was estimated to be between 4000 and 7000.