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The catabolism of cholesterol in vitro. Formation of 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanoic acid from cholesterol by rat liver

Mendelsohn, D.; Mendelsohn, L.

Biochemical Journal 114(1): 1-3

1969


ISSN/ISBN: 0264-6021
PMID: 5810058
DOI: 10.1042/bj1140001
Accession: 044570824

1. Both 25-d- and 25-l-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoic acid were isolated from the gall-bladder bile of Crocodylus niloticus. 2. The catabolism of cholesterol to 25-d- and 25-l-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoic acid respectively was studied by using a rat liver preparation in vitro. The results show that rat liver can metabolize cholesterol to both forms of 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoic acid. However, a preference was noted for the formation from [4-(14)C]cholesterol of 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoic acid (25-d), which was isolated from the incubations with a specific radioactivity about four times that of 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoic acid (25-l). 3. The results indicate that 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoic acid is a normal intermediate in the biosynthesis of bile acids from cholesterol in the rat.

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