+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The cell cycle dependence of thermotolerance. III. HeLa cells heated at 45.0 degrees C



The cell cycle dependence of thermotolerance. III. HeLa cells heated at 45.0 degrees C



Radiation Research 104(3): 429-442



We have extended our studies on the cell cycle dependence of thermotolerance to include HeLa cells heated at 45.0 degrees C to compare the results to Chinese hamster ovary (CHO) cells. We found that asynchronous HeLa cells were more resistant to heat than CHO cells but showed a similar development and decay of thermotolerance. Flow cytometry (FCM) was used to study redistributions in the cell cycle after an initial heat dose. Cells heated for 35 min at 45.0 degrees C were delayed in G1 by about 7 h compared to controls, with delays in late S and G2/M phase also. The heat sensitivity varied through the cell cycle; G1 cells were the most resistant to heat, while S-phase cells were uniformly sensitive throughout S phase, and G2 cells were resistant. Thermotolerance could be induced and expressed in early or late S-phase cells, but to a lesser extent than for G1 cells. The results were similar in many respects to CHO cells, but there were significant differences.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 044571239

Download citation: RISBibTeXText

PMID: 4080985


Related references

The Cell Cycle Dependence of Thermotolerance: Iii. HeLa Cells Heated at 45.0C. Radiation Research 104(3): 429-442, 1985

The cell cycle dependence of thermotolerance. II. CHO cells heated at 45.0 degrees C. Radiation Research 98(3): 491-505, 1984

The cell cycle dependence of thermotolerance. I. CHO cells heated at 42 degrees C. Radiation Research 93(1): 93, 1983

The Cell Cycle Dependence of Thermotolerance: Ii. Cho Cells Heated at 45.0C. Radiation Research 98(3): 491-505, 1984

The Cell Cycle Dependence of Thermotolerance: I. Cho Cells Heated at 42C. Radiation Research 93(1): 93-106, 1983

Development of thermotolerance and changes in intracellular pH in CHO cells heated at 45.0 degrees C at pH 6.6. Radiation Research 115(1): 106-114, 1988

Cell cycle variation of Hsp70 levels in HeLa cells at 37 degrees C and after a heat shock. Journal of Cellular Physiology 165(2): 367-375, 1995

Thermotolerance kinetics and growth rate changes in the R1H tumour heated at 43 degrees C. International Journal of Hyperthermia 4(6): 677-686, 1988

Mild thermotolerance induced at 40 degrees C increases antioxidants and protects HeLa cells against mitochondrial apoptosis induced by hydrogen peroxide: Role of p53. Archives of Biochemistry and Biophysics 495(2): 97, 2010

Time-temperature analysis of cell killing of BHK cells heated at temperatures in the range of 43.5 degrees C to 57.0 degrees C. International Journal of Radiation Oncology Biology Physics 19(2): 389-399, 1990

Cell cycle dependent thermotolerance in neuroblastoma cells cell cycle progression delay and dna synthesis. Overgaard, J. Hyperthermic Oncology, Vol. 1. Summary Papers; 4th International Symposium, Aarhus, Denmark, July 2-6, . Xix 870p. Taylor And Francis: London, England; Philadelphia, Pa., Usa. Illus. 119-122, 1984, 1986

Cell-cycle dependence and properties of the HeLa cell DNA polymerase system. Proceedings of the National Academy of Sciences of the United States of America 82(8): 2220-2224, 1985

Non-apoptotic cell death in heated HeLa S3 cells Ultrastructural observations. Modern Pathology 8(1): 142A, 1995

Visualisation of cell cycle modifications by X-ray irradiation of single HeLa cells using fluorescent ubiquitination-based cell cycle indicators. Radiation Protection Dosimetry 166(1-4): 91-94, 2015

Expression of the mitochondrial genome in hela cells part 9 replication of mitochondrial dna in relationship to the cell cycle in hela cells. Journal Of Molecular Biology: 465-484, 1972