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The electrophoresis of transferrins in urea/polyacrylamide gels






Biochemical Journal 189(3): 541-546

The electrophoresis of transferrins in urea/polyacrylamide gels

The denaturation of transferrin by urea has been studied by (a) electrophoresis in polyacrylamide gels incorporating a urea gradient, (b) measurements of the loss of iron-binding capacity and (c) u.v. difference spectrometry. In human serum transferrin and hen ovotransferrin the N-terminal and C-terminal domains of the iron-free protein were found to denature at different urea concentrations.


Accession: 044632370

PMID: 7213345

DOI: 10.1042/bj1890541



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