The importance of the position of the adenosine linkage in NAD+ for its coenzyme activity via lactate dehydrogenase
Schlimme, E.; Mattern, R.; Schott, E.
Zeitschrift für Naturforschung. Section C Biosciences 37(3-4): 342-344
ISSN/ISBN: 0341-0382 PMID: 7072317 Accession: 044658344
We report on the chemical preparation of the NAD+ analog P1, P2-(5'-beta-nicotinamideribofuranosyl-3"-adenosyl)-diphosphate (N3"AD+) by applying the 1,1'-carbonyldiimidazole activation method. The importance of how adenosine is linked to the nicotinamide mononucleotide phosphoriboride (NMNPR+) moiety is discussed with respect to the coenzyme activity in the lactate dehydrogenase catalyzed reaction.