+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The influence of cultivation on (pro-)insulin biosynthesis and secretion of isolated pancreatic islets of C57BL-mice, long-term treated with glibenclamide in vivo



The influence of cultivation on (pro-)insulin biosynthesis and secretion of isolated pancreatic islets of C57BL-mice, long-term treated with glibenclamide in vivo



Endokrinologie 76(3): 357-364



Long-term treatment of C57Bl/KsJ and C57Bl/6J mice with glibenclamide in vivo caused a diminished response of insulin to high glucose concentrations (20 mmol/l). Islets of those mice were investigated to answer the question whether it is possible to overcome this diminution of glucose sensitivity by cultivation in presence of high glucose (20 mmol/l). The insulin release of islets of glibenclamide treated mice was significantly lowered also in the first 48 h of cultivation. In the following short-term incubation (2 h) no differences in the glucose stimulated insulin secretion could be seen. The second cultivation period (48-96 h) confirmed these results. Both groups of islets (controls and glibenclamide treated) reached comparable values in insulin release. Cultivation of islets of glibenclamide treated mice in presence of 20 mmol/l glucose for at least 2 days led to a restoration of the glucose sensitivity of insulin release. Insulin biosynthesis, judged by measuring 3H-leucine incorporation into (pro-)insulin, was largely unaffected. In all experimental conditions the insulin content was comparable to that of controls.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 044663042

Download citation: RISBibTeXText

PMID: 6781881


Related references

Long term actions of sulfonyl ureas on pro insulin biosynthesis and secretion part 1 lack of evidence for a compensatory increase in pro insulin biosynthesis after exposure of isolated pancreatic rat islets to tolbutamide and glibenclamide in vitro. Hormone and Metabolic Research 9(6): 457-465, 1977

Long-term actions of sulfonylureas on (Pro-) insulin biosynthesis and secretion. III. Functional state of isolated pancreatic rat islets after culture in the presence of tolbutamide and glibenclamide. Hormone and Metabolic Research 13(7): 411-412, 1981

Long term actions of sulfonyl ureas on pro insulin biosynthesis and secretion 3. functional state of isolated pancreatic rat islets after culture in the presence of tolbutamide and glibenclamide. Hormone & Metabolic Research 13(7): 411-412, 1981

Influence of glibenclamide on the pro insulin biosynthesis and secretion in isolated pancreatic islets of c 57bl ksj and c 57bl 6j mice. Diabetologia 15(3): 283, 1978

Hypophysis and function of pancreatic islets. I. The influence of hypophysectomy on insulin secretion and biosynthesis of proinsulin and insulin in isolated pancreatic islets of rats. Diabetologia 9(2): 135-139, 1973

Insulin secretion from isolated pancreatic islets in the female rat short term and long term estradiol influence. Journal de Physiologie 75(3): 289-296, 1979

Insulin secretion from isolated pancreatic islets in the female rat. Short and long term oestradiol influence. Journal de Physiologie 75(3): 289-295, 1979

Changes in glucose-stimulated insulin secretion after long-term treatment of C57BL mice with glibenclamide. Endokrinologie 76(2): 163-170, 1980

Lack of long-term -cell glucotoxicity in vitro in pancreatic islets isolated from two mouse strains (C57Bl/6J; C57Bl/KsJ) with different sensitivities of the -cells to hyperglycaemia in vivo. Journal of Endocrinology 136(2): 289-296, 1993

Insulin biosynthesis and secretion of isolated pancreatic islets of mice after the administration of anti-insulin serum of guinea pigs. Experimental and Clinical Endocrinology 84(1): 87-90, 1984

Hypophysis and function of pancreatic islets. II. The effect of substitution with growth hormone and corticotrophin on insulin secretion and biosynthesis of proinsulin and insulin in isolated pancreatic islets of hypophysectomized rats. Diabetologia 9(2): 140-144, 1973

Impaired insulin secretion in vivo but enhanced insulin secretion from isolated islets in pancreatic beta cell-specific vascular endothelial growth factor-A knock-out mice. Diabetologia 50(2): 380-389, 2007

Lack of long-term beta-cell glucotoxicity in vitro in pancreatic islets isolated from two mouse strains (C57BL/6J; C57BL/KsJ) with different sensitivities of the beta-cells to hyperglycaemia in vivo. Journal of Endocrinology 136(2): 289-296, 1993

Insulin secretion and (pro)insulin biosynthesis of cultured mice islets after glibenclamide loading in vitro. Experientia 36(4): 467-468, 1980

Chronic exposure to glibenclamide impairs insulin secretion in isolated rat pancreatic islets. Journal of Endocrinological Investigation 14(4): 287-291, 1991