+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The isolation of a human Ig V lambda gene from a recombinant library of chromosome 22 and estimation of its copy number



The isolation of a human Ig V lambda gene from a recombinant library of chromosome 22 and estimation of its copy number



Nucleic Acids Research 12(17): 6647-6661



We report the first isolation and characterisation of a human Ig V lambda gene. The gene was isolated from a recombinant phage library of human chromosome 22 using a mouse Ig lambda cDNA as probe. DNA sequence analysis predicts a short leader peptide interrupted by an intron of 88 nucleotides, and a mature polypeptide of 96-97 amino acids which shares 61% homology with mouse V lambda I chains. Comparison with the amino acid sequence of known human lambda chains of all six subgroups shows agreement at 22/25 low variance positions. However the maximum homology with human chains is 49%, so we conclude that this sequence represents a new IgV lambda subgroup. The coding region is followed by the conserved heptamer, CACAGTG, and nonamer, ACATAAACC, sequences which have been implicated in V-J segment recombination. This gene has the hallmarks of an active V lambda gene including recently identified transcriptional controlling sequences. Probing genomic DNA with the subcloned V lambda gene detects a family of about 10 cross hybridizing members at low stringency and 2 at high stringency. There is limited polymorphism of the V lambda locus.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 044671979

Download citation: RISBibTeXText

PMID: 6091030


Related references

The isolation of a human Ig V gene from a recombinant library of chromosome 22 and estimation of its copy number. Nucleic Acids Research 12(17): 6647-6661, 1984

Isolation of six single copy dna sequences from a human chromosome 22 specific library. Cytogenetics & Cell Genetics 46(1-4): 662, 1987

Resistance of Leishmania infantum to allopurinol is associated with chromosome and gene copy number variations including decrease in the S-adenosylmethionine synthetase (METK) gene copy number. International Journal for Parasitology. Drugs and Drug Resistance 8(3): 403-410, 2018

Isolation of single-copy human genes from a library of yeast artificial chromosome clones. Science 244(4910): 1348-1351, 1989

Gene copy-number variation and associated polymorphisms of complement component C4 in human systemic lupus erythematosus (SLE): low copy number is a risk factor for and high copy number is a protective factor against SLE susceptibility in European Americans. American Journal of Human Genetics 80(6): 1037-1054, 2007

Isolation and characterization of a novel low copy number repeated dna sequence from the human x chromosome. American Journal of Human Genetics 39(3 Suppl.): A188, 1986

Copy number estimation algorithms and fluorescence in situ hybridization to describe copy number alterations in human tumors. Pathology International 59(4): 218-228, 2009

High-resolution analysis of gene copy number alterations in human prostate cancer using CGH on cDNA microarrays: impact of copy number on gene expression. Neoplasia 6(3): 240-247, 2004

High-Resolution Analysis of Gene Copy Number Alterations in Human Prostate Cancer Using Cgh on cDna Microarrays: Impact of Copy Number on Gene Expression. Neoplasia 6(3): 240-247, 2004

Isolation of genes from human chromosome 1 1. construction of chromosome 1 gene library. Acta Anatomica Nipponica 59(4): 447, 1984

Isolation of human single copy genomic dna sequences from a chromosome 3 specific library a basis for molecular studies in small cell lung cancer. Proceedings of the American Association for Cancer Research Annual Meeting 28: 52, 1987

Copy number variation arising from gene conversion on the human Y chromosome. Human Genetics 137(1): 73-83, 2018

A chromosome 8 gene-cluster polymorphism with low human beta-defensin 2 gene copy number predisposes to Crohn disease of the colon. American Journal of Human Genetics 79(3): 439-448, 2006

Organization of chicken DNA sequences homologous to the transforming gene of avian myeloblastosis virus. II. Isolation and characterization of lambda proto-amv DNA recombinant clones from a library of leukemic chicken DNA. Journal of Virology 45(3): 925-940, 1983

Evolutionary and functional analysis of RBMY1 gene copy number variation on the human Y chromosome. Human Molecular Genetics 28(16): 2785-2798, 2019