+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The latex agglutination test versus counterimmunoelectrophoresis for rapid diagnosis of bacterial meningitis

The latex agglutination test versus counterimmunoelectrophoresis for rapid diagnosis of bacterial meningitis

Canadian Medical Association Journal 127(6): 489-493

A modified latex agglutination (LA) test was compared with Gram-staining and counterimmunoelectrophoresis (CIE) for the rapid detection in the cerebrospinal fluid (CSF) of antigen to Haemophilus influenzae type b, Neisseria meningitidis groups A, B and C, Escherichia coli K1, Streptococcus pneumoniae and group B streptococci, seven frequent causes of bacterial meningitis in children. Of 50 CSF samples from patients with culture-proven bacterial meningitis 90% were correctly shown by the LA test to contain antigen of the responsible organism. Gram-staining revealed organisms in 80% of 45 of these samples. In 75% of the 40 samples that were of sufficient volume for CIE, positive results for the appropriate antigen were obtained. The concentration of antigen detected in the CSF by the LA test varied from undetectable to 800 000 ng/ml. Patients with a high concentration (more than 2000 ng/ml or a positive result at dilutions of CSF over 1/8) were significantly more likely to have a poor response to therapy (two died and two had persistent pleocytosis or bacteria in the CSF) than patients with a lower concentration (4/16 v. 0/18, P < 0.05). After appropriate therapy was begun the concentration of antigen fell dramatically, but measurable amounts of antigen persisted in the CSF for up to 6 days. The LA test detected bacterial antigen at concentrations 2 to 70 times below the lower limit detected by CIE. In seven additional patients who had received antibiotics before lumbar puncture was performed the LA test detected antigen from meningitis-causing bacteria even though cultures of the CSF were sterile. In another 145 patients who did not have meningitis the results of the LA test were negative. The LA test, done as described in this article, is easier to perform than CIE and should be a useful addition to the diagnostic tests carried out on the CSF of any patient suspected of having meningitis.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 044674328

Download citation: RISBibTeXText

PMID: 6749272

Related references

Evaluation of latex agglutination test in comparison to culture and counterimmunoelectrophoresis in the diagnosis of bacterial meningitis. Revista do Instituto Adolfo Lutz 55(1): 19-24, 1995

Latex agglutination, counterimmunoelectrophoresis, and protein A co-agglutination in diagnosis of bacterial meningitis. Journal of Clinical Pathology 31(12): 1167-1171, 1978

Rapid diagnosis of purulent meningitis by latex agglutination and counterimmunoelectrophoresis. Indian Journal of Pediatrics 49(1): 64-64, 1982

Rapid diagnosis of bacterial meningitis by latex agglutination test. Journal of the Egyptian Public Health Association 64(1-2): 31-44, 1989

Latex Agglutination Test (LAT) For rapid diagnosis of acute bacterial meningitis. Pediatric Infectious Disease 6(4): 150-152, 2014

Latex agglutination test and counterimmunoelectrophoresis in the diagnosis of Haemophilus influenzae meningitis. Pathologie-Biologie 31(2): 97-101, 1983

Comparison of counterimmunoelectrophoresis, latex agglutination and bacterial culture for the diagnosis of bacterial meningitis using urine, serum and cerebrospinal fluid samples. Brazilian Journal of Medical and Biological Research 25(4): 357-367, 1992

Clinical utility of latex agglutination test for the rapid diagnosis of acute bacterial meningitis. Neurology India 66(4): 1050-1051, 2018

Detection of pathogens in bacterial meningitis. Comparison of Gram staining, culture, latex agglutination test and counterimmunoelectrophoresis. Monatsschrift Kinderheilkunde 135(9): 628-631, 1987

Rapid diagnosis of acute bacterial meningitis by coagglutination and latex agglutination. Serodiagnosis and ImmunoTherapy in Infectious Disease 3(5): 317-321, 1989

Use of a latex agglutination test in rapid diagnosis of acute meningitis. Enfermedades Infecciosas Y Microbiologia Clinica 7(4): 186-188, 1989

Rapid identification of the etiological agent by the latex agglutination test in acute bacterial meningitis. Revista Chilena de Pediatria 56(3): 159-161, 1985

Clinical use of the latex agglutination test for rapid diagnosis of purulent meningitis. Ceskoslovenska Pediatrie 44(9): 556-557, 1989

Bacterial meningitis: diagnosis by latex agglutination test and clinical features. Indian Pediatrics 30(4): 495-500, 1993

Latex particle agglutination test as an adjunct to the diagnosis of bacterial meningitis. Indian Journal of Medical Microbiology 25(4): 395-397, 2007