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The preservation of gonococci in liquid nitrogen

, : The preservation of gonococci in liquid nitrogen. Journal of Clinical Pathology 24(2): 122-123

Gonococci suspended in 1% proteose peptone containing 8% glycerol can be snap frozen in liquid nitrogen without detectable loss on thawing. This recovery rate has allowed the use of frozen organisms as the starting inoculum for the bulk growth of gonococci and to preserve gonococci both in urethral pus and after primary subculture for use in studies on gonococcal virulence. The method described for freezing urethral pus on charcoal swabs should make it possible to transport infected specimens from areas lacking adequate laboratory facilities.

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Accession: 044714209

PMID: 4994566

DOI: 10.1136/jcp.24.2.122

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Related references

Storck, H.; Rinderknecht, P.; Fries, K., 1949: The identification of gonococci, of pseudo-gonococci, and of penicillin-resistant gonococci by culture techniques. Technique: cultivation on cooked blood agar (Warren-Crowe) with addition of 20% ascites; incubation under increased CO2 tension for 48 hrs.; differentiation from other diplococci of the Neisseria group by fermentation with various sugars. 280 pati...

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