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The primary and secondary structure of the 5'-end region of encephalomyocarditis virus RNA. A novel approach to sequencing long RNA molecules

, : The primary and secondary structure of the 5'-end region of encephalomyocarditis virus RNA. A novel approach to sequencing long RNA molecules. Gene 26(2-3): 189-195

The primary structure of a 149-nucleotide fragment of encephalomyocarditis (EMC) virus RNA from the 5'-terminus of the genome up to the poly(C) tract (S fragment) has been determined. For isolation of the S fragment, site-directed fragmentation of the viral RNA with RNase H and poly(dG) was employed. For sequencing the S fragment, a novel approach has been developed, which can be used for primary structure determination of long RNA molecules. A model of the secondary structure of the S fragment is proposed, according to which this region of RNA is highly structured. The role of complementary oligonucleotide stretches near both termini of the RNA molecule is discussed.

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Accession: 044715492

PMID: 6323259

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Ramamurthi B.N., 1973: Dermaptera from thailand with descriptions of 3 new species insecta dermaptera. Steenstrupia 3(2): 11-16

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Schultze, A.; Ben Batalla, I.; Riethdorf, S.; Bubenheim, M.; Yekebas, E.; Erbersdobler, A.; Reichelt, U.; Effenberger, K.E.; Schmidt, T.; Izbicki, J.R.; Bokemeyer, C.; Pantel, K.; Fiedler, W.; Loges, S., 2013: VEGFR-1 expression levels predict occurrence of disseminated tumor cells in the bone marrow of patients with esophageal carcinoma. Blocking angiogenesis by inhibiting VEGF represents an established therapeutic strategy in many cancers. The role of placental growth factor (PlGF) and of its receptor VEGFR-1 in tumor biology remain more elusive. Currently, humanized monoclonal a...

Wolinsky, J. S., 1977: Mumps virus induced hydrocephalus in hamsters ultrastructure of the chronic infection. Newborn hamsters were inoculated i.p. with neuroadapted mumps virus and sacrificed at intervals from days 3-50. Intracytoplasmic collections of nucleocapsids were found in ependymal cells and neurons on days 3 and 5 and virus formed at the plasma...

Berger, J., 1983: The effects of repeated bleedings on bone marrow and blood morphology in adult laboratory rats. There were slight and rapidly disappearing changes in the bone marrow and blood morphology of laboratory rats after repeated bleedings on consecutive days, except marked increases in the counts of circulating reticulocytes. However, the significan...

Hobensack, Bertha, 1947: The influence of estrogenic substances upon the irritability of the uterine musculature and upon the cardiovascular system. Federation Proc: 387

Gauthier, S.; Carestia, C.; Scarano, E., 1974: Identification of the products of reaction of endoribonuclease from non-fertilized ova of Asterias forbesii. Bollettino Della Societa Italiana di Biologia Sperimentale 50(23-24): 2033-2035

Kim TaeGyun; Kim SeungHee; Kang SoegYoun; Jung KiKyung; Choi DonHa; Park YongBok; Ryu JongHoon; Han HyungMee, 2000: Antiatherogenic effect of the extract of Allium victorialis on the experimental atherosclerosis in the rabbit and transgenic mouse. Atherosclerosis is emerging as one of the major causes of death in Korea as well as Western societies. In the present study, hypocholesterolaemic and antiatherogenic effects of the ethanol extract of A. victorialis were investigated using the conv...

Manukyan, AR., 1988: Review of the genera Suissaba Cameron and Xestopelta Dasch (Hymenoptera, Ichneumonidae) of the USSR fauna. Trudy Zoologicheskogo Instituta, 175: 44-54

Burckhardt, I; Blau, G., 1977: A decade of economic calculations in dairy farms. Tierzuchter 29 (7) 301-304