+ Translate

The primary structure of hen ovotransferrin

, : The primary structure of hen ovotransferrin. European Journal of Biochemistry 122(2): 297-303

Peptide sequences obtained from hen ovotransferrin are compared with the complete amino acid sequence of the protein deduced from a cDNA sequence (Jeltsch and Chambon, preceding paper). Of the 705 positions of the whole protein 605 can be matched by the peptide sequences. Some possible discrepancies between the two methods are pointed out. The two halves of the chain show marked similarities in their sequences with 37% identical residues. The positions of the 15 disulphide bridges are shown; there are 6 homologous bridges in each half of the molecule and 3 extra bridges which occur only in the C-terminal half. The terminal residues of the half-molecule fragments obtained by limited proteolysis are identified. The two domains are joined by a 9-residue connecting peptide. Sequence variability has been found at 9 positions. The sequence of hen ovotransferrin is compared with the partial available for human transferrin. From this some tentative conclusions about the identities of the metal-binding residues and about the evolution of transferrin are reached.

(PDF 0-2 workdays service)

Accession: 044715694

PMID: 6895872

Submit PDF Full Text: Here

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Related references

Tsao, D.; Azari, P.; Phillips, J.L., 1974: On the structure of ovotransferrin. I. Isolation and characterization of cyanogen bromide fragments. Reevaluation of the primary structure. Biochemistry 13(3): 397-403

Keung, W.M.; Azari, P., 1982: Structure and function of ovotransferrin. II. Iron-transferring activity of iron-binding fragments of ovotransferrin with chicken embryo red cells. The iron-transferring activity of iron-binding fragments FeNF and FeCF, representing the NH2- and CO2H-terminal domains of iron-ovotransferrin (Fe2OT), with a chicken embryo red cell system, was investigated. Each fragment is able to deliver iron...

Keung, W.M.; Azari, P.; Phillips, J.L., 1982: Structure and function of ovotransferrin. I. Production of iron-binding fragments from iron-ovotransferrin by the action of immobilized subtilisin. Purification and characterization of the fragments. Immobilized subtilisin Novo was used for the cleavage of iron-saturated ovotransferrin (Fe2OT) into separate NH2- and CO2H-terminal iron-binding fragments, designated as FeNF and FeCF, respectively. The Mr of each fragment is 39,000. The purified...

Gentili, C.; Doliana, R.; Bet, P.; Campanile, G.; Colombatti, A.; Descalzi Cancedda, F.; Cancedda, R., 1994: Ovotransferrin and ovotransferrin receptor expression during chondrogenesis and endochondral bone formation in developing chick embryo. Ovotransferrin expression during chick embryo tibia development has been investigated in vivo by immunocytochemistry and in situ hybridization. Ovotransferrin was first observed in the 7 day cartilaginous rudiment. At later stages, the factor was...

Ogunnariwo, J.A.; Schryvers, A.B., 1992: Correlation between the ability of Haemophilus paragallinarum to acquire ovotransferrin-bound iron and the expression of ovotransferrin-specific receptors. To investigate the mechanisms of iron acquisition in avian haemophili, strains of Haemophilus paragallinarum and H. avium were tesetd for siderophore production and utilization of transferrin iron for growth. No evidence of siderophore production...

Phillips, J.L.; Azari, P., 1971: On the structure of ovotransferrin. Isolation and characterization of the cyanogen bromide fragments and evidence for a duplicate structure. Biochemistry 10(7): 1160-1165

Abola, J.; Wood, M.; Chweh, A.A.raham, D.P.lsinelli, P., 1982: Structure of hen ovotransferrin at 5 A resolution. Biochemistry and physiology of iron: proceedings Fifth International Conference on Proteins of Iron Storage and Transport Univ of California San Diego August 24-26-1981 editors P Saltman J Hegenauer: 1

Tsao, D.; Azari, P.; Phillips, J.L., 1974: On the sturcture of ovotransferrin. 3. Nitration of iron--ovotransferrin and distribution of tyrosines involved in iron-binding activity. Biochemistry 13(3): 408-413

Jianping Wu; Alexandra Acero-Lopez, 2012: Ovotransferrin Structure, bioactivities, and preparation. Ovotransferrin, accounting for about 12 13% of total egg white proteins, is synthesized by the avian transferrin gene in the oviduct. It is made of a monomeric glycoprotein consisting of 686 amino acid residues. As a member of transferrin family,...

Spik, G.F.urnet, B.M.ntreuil, J., 1979: Study of the structure of the glycan of hen ovotransferrin. Comptes rendus hebdomadaires des seances Serie D Sciences naturelles979 288(11): 967-970