+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The relationship of the chemotactic behavior of the complement-derived factors, C3a, C5a, and C567, and a bacterial chemotactic factor to their ability to activate the proesterase 1 of rabbit polymorphonuclear leukocytes



The relationship of the chemotactic behavior of the complement-derived factors, C3a, C5a, and C567, and a bacterial chemotactic factor to their ability to activate the proesterase 1 of rabbit polymorphonuclear leukocytes



Journal of Experimental Medicine 135(2): 376-387



The inhibition profiles obtained when a series of p-nitrophenyl ethyl alkylphosphonates and of p-nitrophenyl ethyl chloroalkylphosphonates were used to interfere with the chemotactic activity of polymorphonuclear leukocytes stimulated by C3a, C5a, and bacterial factor were the same as found previously when C567 was the chemotactic agent. This indicates that as in the chemotactic activity induced by C567, an obligatory step in the chemotaxis caused by C3a, C5a, and bacterial factor is the activation of proesterase 1 of the rabbit polymorphonuclear leukocyte. C5a and C3a activate proesterase 1 of peripheral blood polymophonuclear leukocytes as measured by the increase of acetyl DL-phenylalanine beta-naphthyl esterase activity. Attempts to detect in a like manner the proesterase 1 of the same leukocytes using bacterial factor under varying circumstances have consistently failed. It is concluded that bacterial factor, for unknown reasons, is unable to activate proesterase 1 to the same extent as the complement-derived chemotactic factors. The hypothesis of there being a quantitative difference in the ability of bacterial factor to activate proesterase 1 compared with the complement-derived factors explains the previous observations that bacterial factor can not deactivate to itself or to the complement-derived factors, although these latter factors can deactivate to themselves, to each other, and to the bacterial factor. The quantitative difference in the ability of bacterial factor to activate proesterase 1 compared to the complement-derived factors is also associated with and explains the finding that the maximal chemotactic activity attainable when bacterial factor is the chemotactic agent is distinctly less than that obtained using either C3a, C5a, or C567. These results indicate that the activation of proesterase 1 is a general requirement for the chemotactic activity of rabbit polymorphonuclear leukocytes with known macromolecular chemotactic agents and suggest that under several different circumstances the level of chemotactic activity attained is related to the degree of such activation.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 044730388

Download citation: RISBibTeXText

PMID: 4551218

DOI: 10.1084/jem.135.2.376


Related references

Normal chemotactic migration of polymorphonuclear leukocytes stimulated with mononuclear-derived chemotactic factor in ulcerative colitis. International Archives of Allergy and Applied Immunology 81(1): 63-68, 1986

Chemotactic effects of the complement-derived peptides C3a, C3ai and C5a (classical anaphylatoxin) on rabbit and guinea-pig polymorphonuclear leukocytes. Naunyn-Schmiedeberg's Archives of Pharmacology 305(2): 181-184, 1978

Generation of polymorphonuclear leukocyte chemotactic activity in rabbit serum and guinea pig serum treated with immune complexes evidence for complement 5a as the major chemotactic factor. Infection & Immunity 1(6): 521-525, 1970

Chemotactic factor to polymorphonuclear leukocytes in the crystallin lens 1. age related chemotactic activity by chemotaxis assay. Nippon Ganka Gakkai Zasshi 89(2): 317-322, 1985

Chemotactic factor to polymorphonuclear leukocytes in the human lens 6. chemotactic factor in low molecular weight fractions. Nippon Ganka Gakkai Zasshi 90(8): 1110-1114, 1986

Chemo taxis of polymorphonuclear leukocytes complement inflammation enz esterase bacterial chemotactic factor cortico steroid immunol chloroquine immunol. Biochemical Pharmacology : 99-105, 1968

Ability of polymorphonuclear leukocytes to orient in gradients of chemotactic factors. Journal of Cell Biology 75(2 Pt 1): 606-616, 1977

The relative responsiveness of mononuclear leukocytes and polymorphonuclear leukocytes to bacterial and serum chemotactic factors. Federation Proceedings 30(2): 355, 1971

Part 1 || Ability of Polymorphonuclear Leukocytes to Orient in Gradients of Chemotactic Factors. Journal of Cell Biology 75(2): 606-616, 1977

Complement c 5 derived chemotactic peptides modulate adherence of polymorphonuclear leukocytes to cultured endothelial cells. Clinical Research 33(2 Part 1): 544A, 1985

Reaction of cobra venom factor with guinea pig complement and generation of an activity chemotactic for poly morphonuclear leukocytes rabbit/ nuclear leukocytes rabbit. Proceedings of the Society for Experimental Biology & Medicine 131(1): 203-207, 1969

Interactions of the complement system with endotoxic lipopolysaccharide. Generation of a factor chemotactic for polymorphonuclear leukocytes. Journal of Experimental Medicine 128(2): 259-275, 1968

Volume changes induced in rabbit polymorphonuclear leukocytes by chemotactic factor and cytochalasin B. American Journal of Pathology 81(1): 1-14, 1975

Complement (C5)-derived chemotactic activity accounts for accumulation of polymorphonuclear leukocytes in cerebrospinal fluid of rabbits with pneumococcal meningitis. Infection and Immunity 46(1): 81-86, 1984

Reaction of a cobra venom factor with guinea pig complement and generation of an activity chemotactic for polymorphonuclear leukocytes. Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine 131(1): 203-207, 1969