The ultrastructure of the spherules of Emmonsia crescens studied by the technique of freeze-etching

Hejtmánek, M.; Necas, O.; Havelková, M.

Mycopathologia et Mycologia Applicata 54(1): 79-83

1974


ISSN/ISBN: 0027-5530
PMID: 4372531
DOI: 10.1007/bf02055976
Accession: 044772406

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Abstract
Young spherules (adiaspores) of the fungus Emmonsia crescens from the cultures growing on Sabouraud's dextrose agar have been prepared for electron microscopy by the technique of freeze-etching. In the cytoplasmic membrane grooved invaginations corresponding with the ridges on the inner face of the cell membrane have been found. The surface of the cytoplasmic membrane is densely covered with regular globules, the diameter of which is about 100 Å. Mitochondria, endoplasmic reticulum, nuclear membrane with pores, and numerous stock materials in the form of fatty drops, and glycogen can be identified in the cytoplasm.

The ultrastructure of the spherules of Emmonsia crescens studied by the technique of freeze-etching