EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

The use of polymerase chain reaction method for the detection of Rickettsia tsutsugamushi in wild rodents


The use of polymerase chain reaction method for the detection of Rickettsia tsutsugamushi in wild rodents



Kansenshogaku Zasshi. Journal of the Japanese Association for Infectious Diseases 69(10): 1103-1109



ISSN/ISBN: 0387-5911

PMID: 7499912

DOI: 10.11150/kansenshogakuzasshi1970.69.1103

We studied the applicability of polymerase chain reaction (PCR) method for the detection of R. tsutsugamushi in wild rodents. The PCR method which amplified the gene coding for the group-specific antigen of R. tsutsugamushi was used in this study. Specific PCR products (88 bp) were obtained with the DNAs from three reference strains (Gilliam, Karp, and Kato) and two cell culture adapted field isolates (KN-1 and GJ-1). The minimum number detectable by the PCR method was estimated to be 1.3 copies of rickettsial genome. In a study with experimentally infected mice, the PCR method could detect rickettsial DNA in one of two infected mice at four months after inoculation. Thereafter, fifty five wild rodents were captured in five areas of Okayama Prefecture, and R. tsutsugamushi DNA was detected, by the PCR method, by amplifying DNA from the spleen of each rodent. The rickettsia was also isolated from the same rodents by the mouse inoculation method. By the PCR method, rickettsia DNAs could be detected in 12 of 13 rodents from which the rickettsiae were isolated, and in 10 of 42 rodents from which no rickettsiae were isolated. These findings indicate that the PCR method is a simple and specific procedure to detect R. tsutsugamushi in wild rodents. On the other hand, the results of the PCR method demonstrated that the middle area of Okayama Prefecture was highly (44-81%) contaminated with R. tsutsugamushi.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 044779133

Download citation: RISBibTeXText

Related references

Survey of antibody on Orientia tsutsugamushi among wild rodents in Gyeongnam area and detection by nested polymerase chain reaction. Korean Journal of Veterinary Research 44(2): 241-249, 2004

Detection of Rickettsia tsutsugamushi DNA by polymerase chain reaction. Nihon Rinsho. Japanese Journal of Clinical Medicine 50 Suppl: 422-426, 1992

Detection of Rickettsia tsutsugamushi DNA from chiggers by polymerase chain reaction. Zhonghua Liu Xing Bing Xue Za Zhi 14(5): 307-309, 1993

Detection of Rickettsia tsutsugamushi specific DNA from the lymphocyte of patients by polymerase chain reaction. Proceedings of the National Science Council Republic of China. Part B Life Sciences 19(1): 43-46, 1995

Detection of Rickettsia tsutsugamushi by gene amplification using polymerase chain reaction techniques. Annals of the New York Academy of Sciences 590: 564-571, 1990

Detection and characterization of Rickettsia tsutsugamushi in infected Leptotrombidium mites using polymerase chain reaction. American Journal of Tropical Medicine and Hygiene 47(4 Suppl. ): 200-201, 1992

Detection of Rickettsia tsutsugamushi from vector trombiculid mite by DNA amplification using polymerase chain reaction technique. Entomologia Sinica 1(4): 322-332, 1994

Detection of Rickettsia tsutsugamushi in chigger mites and patients' blood samples by nested polymerase chain reaction. Chinese Journal of Zoonoses 12(5): 12-15, 1996

ELISA-based colorimetric detection of Rickettsia tsutsugamushi DNA from patient sera by nested polymerase chain reaction. Southeast Asian Journal of Tropical Medicine and Public Health 27(1): 139-144, 1996

Detection of rickettsia tsutsugamushi by polymerase chain reaction pcr amplification of its conserved 47 kda major surface protein antigen gene. Abstracts of the General Meeting of the American Society for Microbiology 92: 111, 1992

Experimental studies on detection of Rickettsia tsutsugamushi in red- and white-body Leptotrombidium deliense by DNA amplification using polymerase chain reaction. Annual Bulletin of the Society of Parasitology Guangdong Province 14(-15): 64-67, 1993

Comparison study of traditional techniques and polymerase chain reaction technique on detection of Rickettsia tsutsugamushi in Leptotrombium deliense. Annual Bulletin of the Society of Parasitology Guangdong Province 14(-15): 68-69, 1993

Trombiculid mites from wild rodents and detection of antibodies against rickettsia tsutsugamushi in the rodents and inhabitants in saga prefecture japan. Medical Entomology and Zoology 39(2): 155-158, 1988

Experiment studies on detection of Rickettsia tsutsugamushi in red-and white-body Leptotrombidium deliense by DNA amplification using polymerase chain reaction technology. Chinese Journal of Zoonoses 10(3): 2-3, 1994

Detection and characterization of Rickettsia tsutsugamushi (Rickettsiales: Rickettsiaceae) in infected Leptotrombidium (Leptotrombidium) fletcheri chiggers (Acari: Trombiculidae) with the polymerase chain reaction. Journal of Medical Entomology 31(5): 691-699, 1994