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Transfer and metabolism of norepinephrine studied from maternal-to-fetal and fetal-to-maternal sides in the in vitro perfused human placental lobe



Transfer and metabolism of norepinephrine studied from maternal-to-fetal and fetal-to-maternal sides in the in vitro perfused human placental lobe



American Journal of Obstetrics and Gynecology 148(4): 474-481



An in vitro system for the dual perfusion of an isolated lobe of human placenta was used to study transfer and metabolism of 3H-labeled norepinephrine at two different concentrations. At 39 mumol/L, the transfer of total radioactivity from the maternal to the fetal side was 11.60% +/- 0.60% and significantly higher (p less than 0.05) than that in the opposite direction (6.50% +/- 0.37%). In both directions almost 50% of tritium was transferred as norepinephrine while the rest was metabolized and appeared on the other side as normetanephrine, dihydroxymandelic acid, and vanillylmandelic acid. When a reduced concentration (870 nmol/L) was infused into either the maternal or the fetal compartment, transfer and metabolism were similar to those determined before. Analysis of the tissue showed 50% of total radioactivity as normetanephrine, dihydroxymandelic acid, and vanillylmandelic acid and 17% as conjugates. Inclusion of the monoamine oxidase inhibitor clorgyline in the perfusate significantly reduced fetal-to-maternal transfer but not transfer in the reverse direction. In addition, inhibition of placental monoamine oxidase resulted in a considerable reduction in dihydroxymandelic acid and vanillylmandelic acid while the normetanephrine fraction increased. Norepinephrine significantly increased glucose utilization and lactate production. A possible physiologic role of norepinephrine transferred from the fetal into the maternal circulation during labor is discussed.

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Accession: 044835148

Download citation: RISBibTeXText

PMID: 6696006

DOI: 10.1016/0002-9378(84)90729-4


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