Trials of in vitro propagation of the scrapie agent in mouse nerve cells
Markovits, P.; Dormont, D.; Delpech, B.; Court, L.; Latarjet, R.
Comptes Rendus des Seances de l'Academie des Sciences. Serie Iii Sciences de la Vie 293(8): 413-417
ISSN/ISBN: 0249-6313 PMID: 6797691 Accession: 044867940
We attempted to propagate the Scrapie agent in vitro in glia and neuroblastoma cells of Mice. Four out of seven assays of infection were positive, i.e. after several passages in vitro yielding at most a 10(9) fold final dilution of the original material, the extracts of each of the four cultures, when injected intracerebrally into CD1 Mice, produced a deadly disease displaying the clinical and pathological signs characteristic of Scrapie.