Home
  >  
Section 45
  >  
Chapter 44,868

Trials of in vitro propagation of the scrapie agent in mouse nerve cells

Markovits, P.; Dormont, D.; Delpech, B.; Court, L.; Latarjet, R.

Comptes Rendus des Seances de l'Academie des Sciences. Serie Iii Sciences de la Vie 293(8): 413-417

1981

ISSN/ISBN: 0249-6313
PMID: 6797691
Accession: 044867940
Download citation:  
Text
  |  
BibTeX
  |  
RIS
We attempted to propagate the Scrapie agent in vitro in glia and neuroblastoma cells of Mice. Four out of seven assays of infection were positive, i.e. after several passages in vitro yielding at most a 10(9) fold final dilution of the original material, the extracts of each of the four cultures, when injected intracerebrally into CD1 Mice, produced a deadly disease displaying the clinical and pathological signs characteristic of Scrapie.

Full Text Article emailed within 1 workday: $29.90




Related References

Markovits, P.; Dormont, D.; Delpech, B.; Court, L.; Latarjet, R. 1981: Experimental in vitro propagation of the scrapie agent in mouse nerve cells Comptes Rendus des Seances de l' Academie des Sciences, III 293(8): 413-417
Markovits, P.; Dautheville, C.; Dormont, D.; Dianoux, L.; Latarjet, R. 1983: In vitro propagation of the scrapie agent. I. Transformation of mouse glia and neuroblastoma cells after infection with the mouse-adapted scrapie strain c-506 Acta Neuropathologica 60(1-2): 75-80
Markovits, P.; Dormont, D.; Dianoux, L.; Latarjet, R. 1983: The in vitro propagation of the causative agent of scrapie modifications of the morphology and growth of mouse neuro blastoma cells nie 115 following inoculation with a cerebral suspension containing the causative agent of scrapie american strain/ Travaux Scientifiques des Chercheurs du Service de Sante des Armees 4: 201-203
Markovits, P.; Dormont, D.; Delpech, B.; Court, L.; Latarjet, R. 1981: Attempts to propagate the scrapie agent in vitro in mouse neural cells Comptes Rendus des Seances de l'Academie des Sciences Serie III Sciences de la Vie 293(8): 413-418
Markovits, P.; Mutel, V.; Dianoux, L.; Launay, J.M.; Haimart, M.; Dormont, D.; Court, L.; Cathala, F. 1985: Effects of in vitro infection of mouse glial and neuroblastoma cells with the scrapie agent Annales de Recherches Veterinaires. Annals of Veterinary Research 16(1): 111-119
Markovits, P.; Dauthville, C.; Dormont, D.; Viret, J.; Davlos, D.; Court, L.; Dianoux, L.; Delpech, B.; Latarjet, R. 1982: In vitro propagation of the scrapie agent Biology of the Cell 45(2): 313
Micco, P. de 1979: Cell fusion induced in vitro by the mouse scrapie agent Comptes Rendus Hebdomadaires des Seances de l' Academie des Sciences 288D(15): 1211-1214
Carp, R.I.; Callahan, S.M. 1981: In vitro Interaction of Scrapie Agent and Mouse Peritoneal Macrophages Intervirology 16(1): 8-13
Carp, R.I.; Callahan, S.M. 1981: In vitro interaction of scrapie agent and mouse peritoneal macrophages Intervirology 16(1): 8-13
De Micco, P. 1979: Cellular fusion induced in vitro by the mouse scrapie agent Comptes Rendus des Seances de l'Academie des Sciences. Serie D Sciences Naturelles 288(15): 1211-1214
Carp, R.I.; Callahan, S.M.; Sersen, E.A.; Moretz, R.C. 1984: Preclinical changes in weight of scrapie-infected mice as a function of scrapie agent-mouse strain combination Intervirology 21(2): 61-69
Vilette, D.; Andreoletti, O.; Archer, F.; Madelaine, M.F.; Vilotte, J.L.; Lehmann, S.; Laude, H. 2001: Ex vivo propagation of infectious sheep scrapie agent in heterologous epithelial cells expressing ovine prion protein Proceedings of the National Academy of Sciences of the United States of America 98(7): 4055-4059
Gibson, P.E.; Bell, T.M.; Field, E.J. 1972: Failure of the scrapie agent to replicate in L5178Y mouse leukaemic cells Research in Veterinary Science 13(1): 95-96
Roikhel, V.M.; Mats, V.N.; Fokina, G.I.; Ravkina, L.I.; Pogodina, V.V. 1983: Reaction of mouse CNS cells to the scrapie agent in early stages of experimental infection Acta Virologica 27(5): 400-406
Markovits, P.; Dormont, D.; Maunoury, R.; Delamarche, L.; Delpech, A.; Dianoux, L.; Latarjet, R. 1982: In vitro modification of the morphology and growth of cells infected with the scrapie agent Comptes Rendus des Seances de l' Academie des Sciences, III 294(7): 305-312
Asher, D.; Yanagihara, R.; Kidson, C.G.jdusek, D.J.; Gibbs, C.J. 1980: Studies of scrapie in cell culture cell-fusing properties of the scrapie and CJD viruses and replication of the scrapie virus in SV 40 transformed mouse brain cells Proceedings International symposium on chronic virus infections: ub 1980) 227-234
Flores-Langarica, A.; Sebti, Y.; Mitchell, D.A.; Sim, R.B.; MacPherson, G.G. 2009: Scrapie pathogenesis: the role of complement C1q in scrapie agent uptake by conventional dendritic cells Journal of Immunology 182(3): 1305-1313
Miyazawa, K.; Masujin, K.; Okada, H.; Ushiki-Kaku, Y.; Matsuura, Y.; Yokoyama, T. 2017: Selective propagation of mouse-passaged scrapie prions with long incubation period from a mixed prion population using GT1-7 cells Plos one 12(6): E0179317
Priola, S.A.; Caughey, B.; Raymond, G.J.; Chesebro, B. 1994: Prion protein and the scrapie agent: in vitro studies in infected neuroblastoma cells Infectious Agents and Disease 3(2-3): 54-58
Rubenstein, R.; Carp, R.I.; Callahan, S.M. 1984: In vitro replication of scrapie agent in a neuronal model: infection of PC12 cells Journal of General Virology 65: 2191-2198