Trimethoprim-induced accumulation of guanosine tetraphosphate (ppGpp) in Escherichia coli

Khan, S.R.; Yamazaki, H.

Biochemical and Biophysical Research Communications 48(1): 169-174

1972


ISSN/ISBN: 0006-291X
PMID: 4557507
DOI: 10.1016/0006-291x(72)90358-0
Accession: 044869840

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Abstract
Trimethoprim, which is known to restrict formylation of methionyl-t RNAf, caused (i) equivalent inhibition of protein accumulation in a rel+ rel + and rel− rel − strain of E. coli E. coli K-12, (ii) severe and rapid inhibition of Rna accumulation in the rel+ rel + strain but a much less severe and more gradual inhibition in the rel− rel − strain, and (iii) most significantly appreciable accumulation of pp Gpp and Ms Ii in the rel+ rel + strain but not in the rel− rel − strain. This observation is consistent with the general notion that whenever formation of aminoacylated t Rna or N-formylmethionyl-t RNAf is restricted in rel+ rel + strains, it evokes pp Gpp accumulation as well as inhibition of Rna accumulation.