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Two-dimensional polyacrylamide gel electrophoresis of acid extractable nuclear proteins of normal rat liver and Novikoff hepatoma ascites cells

Yeoman, L.C.; Taylor, C.W.; Busch, H.

Biochemical and Biophysical Research Communications 51(4): 956-966

1973

ISSN/ISBN: 0006-291X
PMID: 4349999
DOI: 10.1016/0006-291x(73)90020-x
Accession: 044882821
The nuclear proteins of normal rat liver and Novikoff hepatoma nuclei were extracted with 0.4 N H2SO4 and subjected to two-dimensional polyacrylamide gel electrophoresis. A total of 98 protein components were found in the liver extract and 111 components in the tumor extract. A comparison of the patterns obtained revealed 11 qualitative differences and 5 quantitative differences.

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Related References

Yeoman, L.C.; Taylor, C.W.; Jordan, J.J.; Busch, H. 1973: Two-dimensional polyacrylamide gel electrophoresis of chromatin proteins of normal rat liver and Novikoff hepatoma ascites cells Biochemical and Biophysical Research Communications 53(4): 1067-1076
Orrick, L.R.; Olson, M.O.; Busch, H. 1973: Comparison of nucleolar proteins of normal rat liver and Novikoff hepatoma ascites cells by two-dimensional polyacrylamide gel electrophoresis Proceedings of the National Academy of Sciences of the United States of America 70(5): 1316-1320
Busch, G.I.; Yeoman, L.C.; Taylor, C.W.; Busch, H. 1974: Modified two-dimensional polyacrylamide gel electrophoresis systems for higher molecular weight nonhistone chromatin proteins from normal rat liver and Novikoff hepatoma ascites cells Physiological Chemistry and Physics 6(1): 1-10
Prestayko, A.W.; Klomp, G.R.; Schmoll, D.J.; Busch, H. 1974: Comparison of proteins of ribosomal subunits and nucleolar preribosomal particles from Novikoff hepatoma ascites cells by two-dimensional polyacrylamide gel electrophoresis Biochemistry 13(9): 1945-1951
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Willard, K.E.; O'connor T.; Anderson, N.G. 1979: Mapping of chromosomal proteins from rat liver and novikoff hepatoma cells by 2 dimensional electrophoresis Federation Proceedings 38(3 Part 1): 919
Takami, H.; Busch, H. 1979: 2 dimensional gel electrophoretic comparison of proteins of nuclear fractions of normal liver and novikoff hepatoma Cancer Research 39(2 Part 1): 507-518
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Kang, Y.J.; Olson, M.O.J.; Jones, C.E.; Busch, H. 1974: Phosphorylation of nucleolar proteins in isolated nucleoli of novikoff hepatoma ascites cells and normal rat liver Federation Proceedings 33(5 Part 2): 1418
Hirsch, F.W.; Nall, K.N.; Busch, F.N.; Morris, H.P.; Busch, H. 1978: Comparison of abundant cytosol proteins in rat liver, Novikoff hepatoma, and Morris hepatoma by two-dimensional gel electrophoresis Cancer Research 38(6): 1514-1522
Satoh, K.; Busch, H. 1983: Characterization of nucleolar antigens of normal rat liver, regenerating rat liver, and Novikoff ascites hepatoma cells following in vitro translation of polyadenylic acid-containing messenger RNA Cancer Research 43(5): 2143-2149
Wu, B.C.; Spohn, W.H.; Busch, H. 1980: Two-dimensional gel electrophoresis of nuclear phosphoproteins of Novikoff hepatoma and regenerating liver Physiological Chemistry and Physics 12(1): 11-20
Orrick, L.; Olson, M.O.J.; Busch, H. 1973: Comparison of nucleolar proteins of rat liver and novikoff hepatoma by 2 dimensional gel electrophoresis Federation Proceedings 32(3 Part 1): 853
Jones, C.E.; Olson, M.O. 1980: Phosphodipeptide analysis of nonhistone nuclear proteins from Novikoff hepatoma ascites cells International Journal of Peptide and Protein Research 16(2): 135-142
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Goldberg, A.H.; Yeoman, L.C.; Busch, H. 1978: Chromatin-associated glycoproteins of normal rat liver and Novikoff hepatoma ascites cells Cancer Research 38(4): 1052-1056
El-Khatib, S.M.; Ro-Choi, T.S.; Choi, Y.C.; Busch, H. 1970: Studies on nuclear 4.5 S ribonucleic acid IIi of Novikoff hepatoma ascites cells Journal of Biological Chemistry 245(13): 3416-3421
Olson, M.O.; Ezrailson, E.G.; Guetzow, K.; Busch, H. 1975: Localization and phosphorylation of nuclear, nucleolar and extranucleolar non-histone proteins of Novikoff hepatoma ascites cells Journal of Molecular Biology 97(4): 611-619
Reddy, R.; Ro-Choi, T.S.; Henning, D.; Busch, H. 1974: Primary sequence of U-1 nuclear ribonucleic acid of Novikoff hepatoma ascites cells Journal of Biological Chemistry 249(20): 6486-6494
Chen, C.C.; Moore, E.C.; McCall, B.L. 1978: Comparison of thioredoxin reductases from Novikoff ascites hepatoma cells and normal liver of rats Cancer Research 38(7): 1885-1888