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Two-dimensional polyacrylamide gel electrophoresis of chromatin proteins of normal rat liver and Novikoff hepatoma ascites cells

Yeoman, L.C.; Taylor, C.W.; Jordan, J.J.; Busch, H.

Biochemical and Biophysical Research Communications 53(4): 1067-1076

1973

ISSN/ISBN: 0006-291X
PMID: 4356049
DOI: 10.1016/0006-291x(73)90573-1
Accession: 044882823
Chromatin was prepared from the citric acid nuclei of normal rat liver and Novikoff hepatoma ascites cells. After sulfuric acid extraction, the dehistonized chromatin was solubilized by digestion with deoxyribonuclease I. The proteins of normal liver and of Novikoff hepatoma chromatin fractions were analyzed by two-dimensional polyacrylamide gel electrophoresis. The liver pattern contained 69 components and the hepatoma pattern contained 84 components. Comparison of the two patterns revealed two dense protein spots migrating in the B region in the liver pattern that were absent from the tumor pattern and two dense protein spots migrating in the C region in the tumor pattern that were absent from the liver pattern.

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Related References

Busch, G.I.; Yeoman, L.C.; Taylor, C.W.; Busch, H. 1974: Modified two-dimensional polyacrylamide gel electrophoresis systems for higher molecular weight nonhistone chromatin proteins from normal rat liver and Novikoff hepatoma ascites cells Physiological Chemistry and Physics 6(1): 1-10
Orrick, L.R.; Olson, M.O.; Busch, H. 1973: Comparison of nucleolar proteins of normal rat liver and Novikoff hepatoma ascites cells by two-dimensional polyacrylamide gel electrophoresis Proceedings of the National Academy of Sciences of the United States of America 70(5): 1316-1320
Yeoman, L.C.; Taylor, C.W.; Busch, H. 1973: Two-dimensional polyacrylamide gel electrophoresis of acid extractable nuclear proteins of normal rat liver and Novikoff hepatoma ascites cells Biochemical and Biophysical Research Communications 51(4): 956-966
Prestayko, A.W.; Klomp, G.R.; Schmoll, D.J.; Busch, H. 1974: Comparison of proteins of ribosomal subunits and nucleolar preribosomal particles from Novikoff hepatoma ascites cells by two-dimensional polyacrylamide gel electrophoresis Biochemistry 13(9): 1945-1951
Goldberg, A.H.; Yeoman, L.C.; Busch, H. 1978: Chromatin-associated glycoproteins of normal rat liver and Novikoff hepatoma ascites cells Cancer Research 38(4): 1052-1056
Willard, K.E.; O'connor T.; Anderson, N.G. 1979: Mapping of chromosomal proteins from rat liver and novikoff hepatoma cells by 2 dimensional electrophoresis Federation Proceedings 38(3 Part 1): 919
Kang, Y.J.; Olson, M.O.J.; Jones, C.E.; Busch, H. 1974: Phosphorylation of nucleolar proteins in isolated nucleoli of novikoff hepatoma ascites cells and normal rat liver Federation Proceedings 33(5 Part 2): 1418
Hirsch, F.W.; Nall, K.N.; Busch, F.N.; Morris, H.P.; Busch, H. 1978: Comparison of abundant cytosol proteins in rat liver, Novikoff hepatoma, and Morris hepatoma by two-dimensional gel electrophoresis Cancer Research 38(6): 1514-1522
Busch, R.K.; Busch, H. 1977: Antigenic proteins of nucleolar chromatin of Novikoff hepatoma ascites cells Tumori 63(4): 347-357
Taylor, C.W.; Yeoman, L.C.; Jordan, J.J.; Goldknopf, I.L.; Busch, H. 1976: Quantitative analysis of the distribution of 3H-leucine in acid soluble chromatin proteins of Noviknoff hepatoma cells separated by two-dimensional polyacrylamide gel electrophoresis Physiological Chemistry and Physics 8(1): 3-12
Orrick, L.; Olson, M.O.J.; Busch, H. 1973: Comparison of nucleolar proteins of rat liver and novikoff hepatoma by 2 dimensional gel electrophoresis Federation Proceedings 32(3 Part 1): 853
Ezrailson, E.G.; Olson, M.O.; Guetzow, K.A.; Busch, H. 1976: Phosphorylation of non-histone chromatin proteins in normal and regenerating rat liver, Novikoff hepatoma and rat heart Febs Letters 62(1): 69-73
Kang, Y.J.; Olson, M.O.; Jones, C.; Busch, H. 1975: Nucleolar phosphoproteins of normal rat liver and Novikoff hepatoma ascites cells Cancer Research 35(6): 1470-1475
Olson, M.O.; Hatchett, S.; Allan, R.; Hawkins, R.C.; Busch, H. 1978: Nucleolus-associated phosphoprotein kinases of normal and regenerating liver and Novikoff hepatoma ascites cells Cancer Research 38(10): 3421-3426
Olson, M.O.J.; Hawkins, T.C. 1977: Nucleolar associated phospho protein kinases of normal rat liver and novikoff hepatoma ascites cells Proceedings of the American Association for Cancer Research 18: 83
Wikman, J.; Quagliarotti, G.; Howard, E.; Choi, Y.C.; Busch, H. 1970: A comparison of 32P distribution in oligonucleotides of ribosomal 28 S RNA from normal liver and Novikoff hepatoma ascites cells Cancer Research 30(11): 2749-2759
Chen, C.C.; Moore, E.C.; McCall, B.L. 1978: Comparison of thioredoxin reductases from Novikoff ascites hepatoma cells and normal liver of rats Cancer Research 38(7): 1885-1888
Seeber, S.; Busch, H. 1971: Comparative oligonucleotide sequences of 28 S ribosomal RNA normal liver and Novikoff hepatoma ascites cells Cancer Research 31(12): 1888-1894
Daskal, I.; Merski, J. 1975: Effects of cyclo heximide on rat liver novikoff hepatoma ascites cells increased nuclear peri chromatin granules and altered cell surfaces Federation Proceedings 34(3): 732
Delaunay, J.; Schapira, G. 1972: Rat liver and hepatoma ribosomal proteins two-dimensional polyacrylamide gel electrophoresis Biochimica et Biophysica Acta 259(2): 243-246