+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Two-dimensional restriction mapping by digestion with restriction endonucleases of DNA in agarose and polyacrylamide gels



Two-dimensional restriction mapping by digestion with restriction endonucleases of DNA in agarose and polyacrylamide gels



Journal of Biochemical and Biophysical Methods 9(2): 153-161



We have studied with a number of bacterial restriction enzymes the conditions for digestion of DNA in agarose and polyacrylamide gels. The restriction endonucleases HpaII, MspI, HaeIII, HindIII, TaqI, HhaI, AluI, BamHI, EcoRI and SalI are capable of digesting DNA in agarose gels of low electroendosmosis and low sulfate concentration. All enzymes, except BamHI, are also capable of digesting DNA in polyacrylamide gels. With this method, rapid two-dimensional restriction mapping of genomes with low and high sequence complexity is possible.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 044882840

Download citation: RISBibTeXText

PMID: 6330196

DOI: 10.1016/0165-022x(84)90006-x


Related references

2 dimensional restriction mapping by digestion with restriction endo nucleases of dna in agarose and poly acrylamide gels. Journal of Biochemical & Biophysical Methods 9(2): 153-162, 1984

Restriction endonuclease mapping of DNA using in situ digestion in two-dimensional gels. Febs Letters 89(2): 267-270, 1978

Rapid mapping of restriction sites of a DNA: restriction of agarose gel and two-dimensional analysis of end-labeled DNA. Plasmid 2(3): 394-402, 1979

Isolation of large molecular weight DNA from agarose gels for further digestion by restriction enzymes. Febs Letters 53(1): 84-86, 1975

Analysis of DNA restriction enzyme digests by two-dimensional electrophoresis in agarose gels. Methods in Molecular Biology 31: 177-186, 1994

Discontinuous polyacrylamide gradient agarose gels resolve a wide range of restriction fragments and optimize the efficiency of nucleic acid transfer. Analytical Biochemistry 173(2): 285-288, 1988

Physical mapping of the restriction fragments obtained from bacteriophage T4 dC-DNA with the restriction endonucleases SmaI, KpnI and BglII. Molecular and General Genetics 172(3): 303-312, 1979

Demonstration of REBASE-assisted restriction mapping to determine the recognition site of unknown restriction endonucleases. Biochemistry and Molecular Biology Education 35(5): 337-341, 2007

Cloning, restriction digestion and DNA labeling of large DNA fragments (greater than or equal to 1 kb) in the presence of remelted SeaPlaque GTG agarose gels. Biotechniques 11(6): 784-6, 788, 790-1, 1991

Isolation of DNA from agarose gels using DEAE-paper. Application to restriction site mapping of adenovirus type 16 DNA. Nucleic Acids Research 8(2): 253-264, 1980

Digestion of dna in agarose with restriction enzymes a new analytical approach allowing 2 dimensional analysis of enzymatic dna methylation of eukaryotic genomes. Hoppe-Seyler's Zeitschrift fuer Physiologische Chemie 363(9): 923, 1982

Long PCR Rapid restriction mapping and selective suppression by restriction digestion. Schaefer, B C Current Innovations in Molecular Biology Series; Gene cloning and analysis: Current innovations 179-190, 1997

Digestion of DNA with restriction endonucleases. Current Protocols in Protein Science Appendix 4: Appendix 4i, 2008

Digestion of DNA with restriction endonucleases. Current Protocols in Neuroscience Appendix 1: Appendix 1m, 2008

Utilization of highly repeated dna sequences as a criterium of complete digestion on agarose gels example of human dna restricted by various endonucleases. Electrophoresis 7(3): 145-147, 1986