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Use of monoclonal antibody in an ELISA test for the detection of antibodies to bovine leukaemia virus



Use of monoclonal antibody in an ELISA test for the detection of antibodies to bovine leukaemia virus



Journal of Virological Methods 6(1): 19-29



A variant of the ELISA technique, involving a monoclonal anti-gp51 antibody yields a highly sensitive method for the detection of bovine leukaemia virus (BLV) antibodies. The gp51 antigen-coated microtitre plates are obtained by incubation of plastic-adsorbed monoclonal antibodies with a non-purified mixture of BLV antigens. Sera to be tested are incubated in the wells of the gp51-coated plates and bound antibodies are revealed by an enzyme-linked antibovine immunoglobulin reagent. This test is as sensitive as liquid phase radioimmunoassay using the same gp51 antigen and thus appears as a highly sensitive, practical, rapid and cheap method for the detection of BLV antibodies.

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Accession: 044923383

Download citation: RISBibTeXText

PMID: 6300156

DOI: 10.1016/0166-0934(83)90064-2


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