+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

A large scale analysis of cDNA in Arabidopsis thaliana: generation of 12,028 non-redundant expressed sequence tags from normalized and size-selected cDNA libraries



A large scale analysis of cDNA in Arabidopsis thaliana: generation of 12,028 non-redundant expressed sequence tags from normalized and size-selected cDNA libraries



Dna Research 7(3): 175-180



For comprehensive analysis of genes expressed in the model dicotyledonous plant, Arabidopsis thaliana, expressed sequence tags (ESTs) were accumulated. Normalized and size-selected cDNA libraries were constructed from aboveground organs, flower buds, roots, green siliques and liquid-cultured seedlings, respectively, and a total of 14,026 5'-end ESTs and 39,207 3'-end ESTs were obtained. The 3'-end ESTs could be clustered into 12,028 non-redundant groups. Similarity search of the non-redundant ESTs against the public non-redundant protein database indicated that 4816 groups show similarity to genes of known function, 1864 to hypothetical genes, and the remaining 5348 are novel sequences. Gene coverage by the non-redundant ESTs was analyzed using the annotated genomic sequences of approximately 10 Mb on chromosomes 3 and 5. A total of 923 regions were hit by at least one EST, among which only 499 regions were hit by the ESTs deposited in the public database. The result indicates that the EST source generated in this project complements the EST data in the public database and facilitates new gene discovery.

(PDF emailed within 0-6 h: $19.90)

Accession: 045066889

Download citation: RISBibTeXText

PMID: 10907847

DOI: 10.1093/dnares/7.3.175


Related references

Generation of a large scale repertoire of Expressed Sequence Tags (ESTs) from normalised rainbow trout cDNA libraries. Bmc Genomics 7: 196, 2006

Porcine transcriptome analysis based on 97 non-normalized cDNA libraries and assembly of 1,021,891 expressed sequence tags. Genome Biology 8(4): R45-R45, 2007

Large-scale identification of expressed sequence tags (ESTs) from Nicotiana tabacum by normalized cDNA library sequencing. Beitraege zur Tabakforschung International 22(2): 114-124, 2006

Generation of expressed sequence tags from 21 randomly selected cDNA clones derived from a bovine ovary cDNA library. Animal Genetics 27(SUPPL 2): 94, 1996

Efficient construction of cDNA microarrays utilizing normalized cDNA libraries of Arabidopsis thaliana. Journal of Bioscience and Bioengineering 97(1): 85-88, 2005

Generation and analysis of expressed sequence tags from Trypanosoma cruzi trypomastigote and amastigote cDNA libraries. Molecular and Biochemical Parasitology ust; 136(2): 221-225, 2004

Generation and analysis of large-scale expressed sequence tags (ESTs) from a full-length enriched cDNA library of porcine backfat tissue. Bmc Genomics 7: 36, 2006

Generation and analysis of a large-scale expressed sequence tags from a full-length enriched cDNA library of Siberian tiger (Panthera tigris altaica). Gene 541(2): 75-81, 2014

Generation and analysis of expressed sequence tags from a normalized cDNA library of young leaf from Ma bamboo (Dendrocalamus latiflorus Munro). Plant Cell Reports 30(11): 2045-2057, 2012

Genomics of hybrid poplar (Populus trichocarpa x deltoides) interacting with forest tent caterpillars (Malacosoma disstria): normalized and full-length cDNA libraries, expressed sequence tags, and a cDNA microarray for the study of insect-induced defences in poplar. Molecular Ecology 15(5): 1275-1297, 2006

Expressed sequence tags from normalized cDNA libraries prepared from gill and hypodermal tissues of the blue crab, Callinectes sapidus. Comparative Biochemistry and Physiology. Part D, Genomics and Proteomics 1(2): 200-208, 2006

Generation of expressed sequence tags from a normalized porcine skeletal muscle cDNA library. Animal Biotechnology 13(2): 211-222, 2003

Generation of expressed sequence tags from cDNA libraries of Italian ryegrass (Lolium multiflorum Lam.). Grassland Science 49(6): 593-598, 2004

Generation of expressed sequence tags from mouse eye cDNA libraries for developing custom gene microarrays. IOVS 42(4): S714, March 15, 2001

Generation of expressed sequence tags for mouse liver genes using unfractionated cDNA primary libraries constructed in M13. Hepatology 16(4 PART 2): 189A, 1992