+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

A new biotyping method for Streptococcus mutans with the API ZYM system



A new biotyping method for Streptococcus mutans with the API ZYM system



Clinical Microbiology and Infection 5(2): 88-91



OBJECTIVE: To test a new system for the biotyping of Streptococcus mutans, based on the measurement of enzyme activity, and to investigate the relationship between biotype and in vitro susceptibility to seven clinically useful antibiotics. METHODS: In total, 160 oral isolates of S. mutans were classified into different biotypes with the APIZYM test for enzyme activity, excluding results that were positive or negative in >80% of the strains. The susceptibility of all 160 strains to amoxycillin, cefazolin, erythromycin, clindamycin, vancomycin, teicoplanin and imipenem was tested by dilution in a solid medium. Statistical analysis of susceptibility (mean minimum inhibitory concentrations (MICs)) was based on chi-squared tests. RESULTS: Eight different biotypes (1-8) were identified on the basis of three kinds of enzyme activity: valine aryl amidase, acid phosphatase and alpha-galactosidase. Biotype 5 was found to be the most common. The mean MIC values showed strains belonging to biotype 4 to be the most susceptible to amoxycillin, cefazolin and erythromycin, whereas biotype 1 was the least susceptible to teicoplanin. CONCLUSIONS: The proposed biotyping method, which is relatively fast and simple to perform, provided reproducible results, and may contribute to clinically effective treatment of S. mutans infections.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 045079094

Download citation: RISBibTeXText

PMID: 11856223


Related references

The usefulness of biotyping in the determination of selected pathogenicity determinants in Streptococcus mutans. Bmc Microbiology 14: 194, 2015

Isolation and biotyping of Streptococcus mutans among preschool children in Kaohsiung city. Zhonghua Ya Yi Xue Hui Za Zhi 9(1): 26-32, 1990

Cross reactivity between antibodies against streptococcus mutans 6715 ribosomes and other strains of streptococcus mutans other species of streptococcus and a strain of escherichia coli. Abstracts of the Annual Meeting of the American Society for Microbiology 82: ABSTRACT E63, 1982

The effect of immunization with streptococcus mutans on the development of streptococcus mutans streptococcus sanguis and dental caries in rhesus monkeys. Journal of Dental Research 55(SPEC ISSUE D): D121, 1976

Mixed continuous cultures of Streptococcus mutans with Streptococcus sanguis or with Streptococcus oralis as a model to study the ecological effects of the lactoperoxidase system. Caries Research 27(1): 26-30, 1993

Evaluation of the Vitek system in the identification and biotyping of Streptococcus agalactiae. Enfermedades Infecciosas Y Microbiologia Clinica 9(2): 98-99, 1991

A method for gentle lysis of Streptococcus sanguis and Streptococcus mutans. Biochemical and Biophysical Research Communications 65(1): 378-384, 1975

Method and kit for detection or quantification of streptococcus mutans and streptococcus sorbrinus. Official Gazette of the United States Patent & Trademark Office Patents 1169(3): 1697, Dec 20, 1994

Is Streptococcus mutans really the main enemy? Investigations of Greenland's bacterial flora perhaps put to question the connection between Streptococcus mutans and caries. Tandlaegebladet 79(17): 692-693, 1975

Phosphorus translocation between enamel and streptococcus mutans in the presence of sucrose and fluoride with observations on the acid phosphatase of streptococcus mutans. Caries Research 14(5): 248-257, 1980

Salivary immunoglobulin A antibodies and recovery from challenge of Streptococcus mutans after oral administration of Streptococcus mutans vaccine in humans. Infection and Immunity 39(2): 514-519, 1983

Heart-reactive antibodies in rabbit anti-Streptococcus mutans sera fail to cross-react with Streptococcus mutans. Journal of Immunology 140(3): 954-961, 1988

The relationship between IgA antibodies to Streptococcus mutans antigens in human saliva and breast milk and the numbers of indigenous oral Streptococcus mutans. Archives of Oral Biology 32(1): 21-25, 1987

Streptococcus mutans approximal plaque index as a new epidemiologic tool for defining the parameters of Streptococcus mutans infection in human populations. Archives of Oral Biology 26(5): 345-355, 1981