+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

A new cationic liposome DNA complex enhances the efficiency of arterial gene transfer in vivo

A new cationic liposome DNA complex enhances the efficiency of arterial gene transfer in vivo

Human Gene Therapy 7(15): 1803-1812

An important goal of gene therapy for cardiovascular diseases and cancer is the development of effective vectors for catheter-based gene delivery. Although adenoviral vectors have proven effective for this purpose in animal models, the ability to achieve comparable gene transfer with nonviral vectors would provide potentially desirable safety and toxicity features for clinical studies. In this report, we describe the use of a new cationic DNA-liposome complex using an improved expression vector and lipid, N-(3-aminopropyl)-N, N-dimethyl-2,3-bis(dodecyloxy)-1-propaniminium bromide/dioleyl phosphatidylethanolamine (GAP-DL-RIE/DOPE) to optimize catheter-mediated gene transfer in porcine arteries. The efficiency of this vector was compared to DNA alone, DNA with a previously described cationic liposome complex, (+/-)-N-(2-hydroxyethyl)-N, N-dimethyl-2,3-bis(tetradecyloxy)-1-propanaminium bromide (DMRIE/DOPE), and a replication-defective adenoviral vector in a porcine artery gene transfer model. When used in optimal ratios, GAP-DL-RIE/DOPE liposomes provided a 15-fold higher level of gene expression in arteries compared to DNA alone or DMRIE/DOPE. Gene expression was observed in intimal and medial cells. However, when compared to adenoviral vectors (10(10) pfu/ml), gene expression following GAP-DLRIE/DOPE transfection was approximately 20-fold lower. Following intravenous injection of GAP-DLRIE/DOPE in mice, biochemical, hematological, and histopathological abnormalities were not observed. Significant improvements in the efficacy of arterial gene expression can be achieved by optimization of transfection condition with DNA-liposome complexes in vivo that may prove useful for arterial gene delivery in cardiovascular diseases and cancer.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 045079293

Download citation: RISBibTeXText

PMID: 8894672

DOI: 10.1089/hum.1996.7.15-1803

Related references

Cytoplasmic gene expression system enhances the efficiency of cationic liposome-mediated in vivo gene transfer into mouse brain. Biochemical and Biophysical Research Communications 234(1): 15-18, 1997

In vivo gene transfer into the adult mammalian central nervous system by continuous injection of plasmid DNA-cationic liposome complex. Brain Research 780(1): 119-128, 1998

Protamine-derived synthetic peptide enhances the efficiency of sperm-mediated gene transfer using liposome-peptide-DNA complex. Journal of Reproduction and Development 48(3): 281-286, 2002

High efficiency targeted in vivo gene transfer to kidney tumors using intraarterial administration of plasmid DNA in complex with cationic polyliposome. Journal of Urology 161(4 Suppl. ): 142, 1999

Cationic liposome-mediated, in vivo gene transfer and expression. Journal of Cellular Biochemistry Suppl. 0(21A): 358, 1995

Gene transfer in hematopoietic cells using a new cationic liposome, GLB 43 Transfection efficiency evaluated by three methods. Blood. 86(10 Suppl. 1): 998a, 1995

Evaluation and optimization of different cationic liposome formulations for in vivo gene transfer. Biochemical and Biophysical Research Communications 221(1): 169-173, 1996

Increased efficiency of cisplatin-resistant cell lines to DNA-mediated gene transfer with cationic liposome. Journal of Obstetrics and Gynaecology Research 31(5): 368-374, 2005

Intratumoral pharmacokinetics and in vivo gene expression of naked plasmid DNA and its cationic liposome complexes after direct gene transfer. Cancer Research 57(13): 2681-2686, 1997

Cationic liposome-mediated gene transfer to tumor cells in vitro and in vivo. Methods in Molecular Medicine 7: 329-337, 1997

Characterization of cationic liposome-mediated gene transfer in vivo by intravenous administration. Human Gene Therapy 8(13): 1585-1594, 1997

Efficacy of cationic liposome-mediated gene transfer to mesangial cells in vitro and in vivo. Journal of Molecular Medicine 79(4): 184-189, 2001

Cationic liposome-mediated gene transfer to rat salivary epithelial cells in vitro and in vivo. Journal of Gene Medicine 3(1): 82-90, 2001

Protamine sulfate provides enhanced and reproducible intravenous gene transfer by cationic liposome/DNA complex. Journal of Liposome Research 7(2-3): 207-219, 1997

Cationic liposome-mediated gene transfer to lungs of normal and pulmonary hypertensive mice in vivo. FASEB Journal 9(4): A857, 1995