+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

A new chemical formulation for control of dental unit water line contamination: An 'in vitro' and clinical 'study'



A new chemical formulation for control of dental unit water line contamination: An 'in vitro' and clinical 'study'



Bmc Oral Health 2(1): 1



BACKGROUND: Water delivered by dental units during routine dental practice is highly contaminated. The aim of this study is to evaluate the efficacy of a new chemical solution flushed through Dental Unit Water Lines (DUWL) for the control of contamination inside dental units. MATERIALS AND METHODS: Six old dental units equipped with a device designed to automatically flush disinfecting solutions through the water system (Castellini Autosteril) were selected. Water samples from DUWL effluents were collected in each dental unit for 10 randomly selected days, before and after a 5 minute DUWL disinfecting cycle with TetraAcetylEthileneDiamine (TAED) and persalt (Ster4spray produced by Farmec spa, and distributed by Castellini spa). Water samples were plated in R2A Agar and cultured at room temperature for 7 days, and the total number of heterotrophic microorganisms counted and expressed in Log10 CFU/mL A general linear model was fitted and multiple regression ANOVA for repeated measures was used for the statistical analysis. RESULTS: The mean contamination in DUWL effluent at baseline was 5.45 &#PlusMinus; 0.35 CFU/mL (range 4.79 to 5.93 CFU/mL). When water samples were tested "in vitro" against the chemical, no growth of heterotrophic bacteria was detected after a 5 minute contact in any of the water samples tested. After undergoing a 5 minute disinfecting cycle with the chemical, DUWL mean contamination in water effluents was 2.01 &#PlusMinus; 0.32 CFU/mL (range 1.30 to 2.74 CFU/mL) (significant difference with respect to baseline). CONCLUSIONS: An inbetween patient disinfecting procedure consisting of flushing DUWL with TAED and persalt equivalent to 0.26% peracetic acid could be useful in routine dental practice for cross-contamination control.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 045079354

Download citation: RISBibTeXText

PMID: 11872153

DOI: 10.1186/1472-6831-2-1


Related references

Legionella pneumophila contamination of a dental unit water line system in a dental teaching centre. International Journal of Dental Hygiene 6(1): 48-55, 2008

Disinfection of dental unit water line using aloe vera: in vitro study. International Journal of Dentistry 2013: 618962, 2013

Detection and quantification of dental unit water line contamination by oral streptococci. Infection Control and Hospital Epidemiology 27(5): 504-509, 2006

Comparison of the efficacies of disinfectants to control microbial contamination in dental unit water systems in general dental practices across the European Union. Applied and Environmental Microbiology 72(2): 1380-1387, 2006

Contamination of a dental unit water line system by legionella pneumophila in the mashhad school of dentistry in 2009. Iranian Red Crescent Medical Journal 14(6): 376-378, 2012

In vitro modeling of dental water line contamination and decontamination. Fems Microbiology Letters 235(2): 363-367, 2004

Evaluation of an automated dental unit water system's contamination control protocol. Journal of Contemporary Dental Practice 13(1): 1-10, 2012

Control of microbial contamination in dental unit water systems using tetra-sodium EDTA. Journal of Applied Microbiology 107(4): 1081-1088, 2010

Failure of anti-retraction valves and the procedure for between patient flushing: a rationale for chemical control of dental unit waterline contamination. American Journal of Dentistry 18(4): 270-274, 2005

Cross-infection in dentistry: an appraisal of the control guidelines with regard to contamination of dental unit water systems. Singapore Dental Journal 18(1): 6-8, 1993

A between-patient disinfection method to control water line contamination and biofilm inside dental units. Journal of Hospital Infection 56(4): 297-304, 2004

A pilot study of three methods for the reduction of bacterial contamination of dental unit water systems in routine use. International Journal of Hygiene and Environmental Health 204(5-6): 303-308, 2002

Effective control of dental chair unit waterline biofilm and marked reduction of bacterial contamination of output water using two peroxide-based disinfectants. Journal of Hospital Infection 52(3): 192-205, 2002

Microbiological evaluation of a range of disinfectant products to control mixed-species biofilm contamination in a laboratory model of a dental unit water system. Applied and Environmental Microbiology 69(6): 3327-3332, 2003

Microbial biofilm formation and contamination of dental-unit water systems in general dental practice. Applied and Environmental Microbiology 66(8): 3363-3367, 2000