+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

A new chromogenic substrate for angiotensin-converting enzyme

A new chromogenic substrate for angiotensin-converting enzyme

Analytical Biochemistry 91(2): 674-683

The compound para-nitrobenzyloxycarbonylglycyl-(S-4-nitrobenzo-2-oxa- 1,3-diazole)-L-cysteinylglycine [NO2ZGly(S-NBD)CysGly] with an absorption maximum at 423 nm is readily hydrolyzed by angiotensin-converting enzyme (EC peptidlyldipeptide hydrolase) to yield the S-benzfurazan derivative of cysteinylglycine. An internal S-->N shift occurs immediately to yield the N-benzfurazan derivative which in turn reacts with the sulfhydryl reagent 4,4'-dithiodipyridine to produce the mixed disulfide with an intense absorption at 461 nm. The maximum difference in molar absorptivity of 13,000 M-1 cm-1 occurs at 470 nm.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 045079368

Download citation: RISBibTeXText

PMID: 9762154

DOI: 10.1016/0003-2697(78)90554-7

Related references

Murine Recombinant Angiotensin-Converting Enzyme 2 Effect on Angiotensin II-Dependent Hypertension and Distinctive Angiotensin-Converting Enzyme 2 Inhibitor Characteristics on Rodent and Human Angiotensin-Converting Enzyme 2 Renin-Angiotensin-Aldosteron. 2012

Murine recombinant angiotensin-converting enzyme 2: effect on angiotensin II-dependent hypertension and distinctive angiotensin-converting enzyme 2 inhibitor characteristics on rodent and human angiotensin-converting enzyme 2. Hypertension 60(3): 730-740, 2013

Angiotensin-converting enzyme inhibition attenuates lipopolysaccharide-induced lung injury by regulating the balance between angiotensin-converting enzyme and angiotensin-converting enzyme 2 and inhibiting mitogen-activated protein kinase activation. Shock 43(4): 395-404, 2016

Aerobic exercise training-induced left ventricular hypertrophy involves regulatory MicroRNAs, decreased angiotensin-converting enzyme-angiotensin ii, and synergistic regulation of angiotensin-converting enzyme 2-angiotensin (1-7). Hypertension 58(2): 182-189, 2011

Vasoconstrictor effect of the angiotensin converting enzyme resistant chymase substrate angiotensin I in human dorsal hand veins In vivo demonstration of non ACE conversion of angiotensin II in humans. Journal of the American College of Cardiology 37(2 Supplement A): 275A, 2001

Angiotensin-converting enzyme inhibition and angiotensin AT1 receptor blockade downregulate angiotensin-converting enzyme expression and attenuate renal injury in streptozotocin-induced diabetic rats. Journal of Biochemical and Molecular Toxicology 27(7): 378-387, 2013

Vasoconstrictor effect of the angiotensin-converting enzyme-resistant, chymase-specific substrate [Pro(11)(D)-Ala(12)] angiotensin I in human dorsal hand veins: in vivo demonstration of non-ace production of angiotensin II in humans. Circulation 104(15): 1805-1808, 2001

Angiotensin-Converting Enzyme-2 (ACE2)--A New Player in the Genesis of Glomerular Injury?: Loss of Angiotensin-Converting Enzyme-2 Leads to the Late Development of Angiotensin II-Dependent Glomerulosclerosis. Am J Pathol 168: 1808-1820, 2006. Journal of the American Society of Nephrology 17(10): 2637-2643, 2006

Effect of reduced angiotensin-converting enzyme gene expression and angiotensin-converting enzyme inhibition on angiotensin and bradykinin peptide levels in mice. Hypertension 43(4): 854-859, 2004

Angiotensin-converting enzyme inhibitor versus angiotensin 2 receptor antagonist therapy and the influence of angiotensin-converting enzyme gene polymorphism in IgA nephritis. Annals of the Academy of Medicine, Singapore 37(5): 372-376, 2008

Formation of angiotensin II from tetradecapeptide renin substrate by angiotensin-converting enzyme. Biochemical Pharmacology 24(10): 1137-1139, 1975

Sensitive fluorimetric assay for serum angiotensin-converting enzyme with the natural substrate angiotensin I. American Journal of Clinical Pathology 68(2): 225-228, 1977

Angiotensin I-converting enzyme inhibition but not angiotensin II suppression alters angiotensin I-converting enzyme gene expression in vessels and epithelia. Journal of Pharmacology and Experimental Therapeutics 284(3): 1180-1187, 1998

Sex-Specific Changes in Renal Angiotensin-Converting Enzyme and Angiotensin-Converting Enzyme 2 Gene Expression and Enzyme Activity at Birth and Over the First Year of Life. Reproductive Sciences 23(2): 200-210, 2017

Inhibition of angiotensin-converting enzyme by des-Leu10-angiotensin I: a potential mechanism of endogenous angiotensin-converting enzyme regulation. Biochimica et Biophysica Acta 871(1): 1-5, 1986