+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

A new coincidence model for single particle counters, Part II: Advances and applications

A new coincidence model for single particle counters, Part II: Advances and applications

Pda Journal of Pharmaceutical Science and Technology 48(5): 255-292

Accuracy, acceptance limits and methods for U.S.P. (788) contaminating particle assays published in the XXII Revision are refined in U.S.P. XXIII. In both Revisions, although different numerical values and methods are employed, particle contamination limits remain constants for all S.V.I. container volumes. The effect of this quality standard is high particle concentration acceptance limits in the smallest S.V.I. container sizes. The effect of these high concentrations is to introduce both undercount errors and false counts into U.S.P. (788) SVI contaminating particle assays. There is general agreement that the count of high concentrations of particles by a single particle light extinction counter result in an increase of the average size of the distribution of particles reported and a decrease in their total number. The error mechanism is termed "signal coincidence." Understanding and control of both these problems is unified with the introduction of the count efficiency parameter. Part I of this paper makes available two core concepts with which evaluation and control of coincidence error in single particle counters can be accurately quantified. These two core concepts are the "Particle Triggered Poisson Model," a new more accurate statistical model of the particle counting process and a concentration measure that includes the effect of particle size on the counting capability of a detector. Use of these concepts make it possible to evaluate particle detector count efficiency capability from experimental data of the coincidence effect. This is an application paper. It combines the theory in the Part I paper with the replicability of particle counters into a simple test protocol. The test results can be used to calculate a contour of particle size and count within which both undercount errors and the introduction of false counts into U.S.P. (788) particle assays are controlled. From the data analyzed it can be seen that any single particle size test cannot effectively evaluate detector performance. The use of the theory and methodology described can help realize the intent of the U.S.P. (788) SVI particle contamination assay.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 045079543

Download citation: RISBibTeXText

PMID: 8000900

Related references

A new coincidence model for single particle counters, part III: realization of single particle counting accuracy. Pda Journal of Pharmaceutical Science and Technology 50(2): 99-122, 1996

A new coincidence model for single particle counters, Part I: Theory and experimental verification. Journal of Pharmaceutical Science and Technology 48(3): 110-134, 1994

Response of single particle optical counters to nonideal particles. Environmental Science & Technology 1(10): 801-814, 1967

Validation of the counting accuracy of particle counters with the new particle counting model. Meltzer, T H, Jornitz, M W Filtration in the biopharmaceutical industry 847-896, 1998

Identification of sources of atmospheric PM at the Pittsburgh Supersite - Part II: Quantitative comparisons of single particle, particle number, and particle mass measurements. Atmospheric Environment 40(Suppl. 2): S424-S444, 2006

Single particle fluorescence: a simple experimental approach to evaluate coincidence effects. Applied Spectroscopy 61(7): 711-718, 2007

Out-of-plane integration of a multimode optical fiber for single particle/cell detection at multiple points on a microfluidic device with applications to particle/cell counting, velocimetry, size discrimination and the analysis of single cell lysate injections. Lab on A Chip (): -, 2016

Correction of coincidence error in counters. Die Naturwissenschaften 56(12): 633-634, 1969

Using proportional counters in coincidence for low background counting. Health Physics 62(6 SUPPL): S45, 1992

Delayed coincidence circuit for scintillation counters. Review of Scientific Instruments 22(5): 324-327, 1951

Recent Advances in Analytical Methods on Lipoprotein Subclasses: Calculation of Particle Numbers from Lipid Levels by Gel Permeation HPLC Using "Spherical Particle Model". Journal of Oleo Science 65(4): 265-282, 2016

Geometric factors underlying coincidence counting with Geiger counters. Review of Scientific Instruments 19(6): 384-389, 1948

Scanning electron microscopy 1976 vol 2 proceedings of the workshops on biological applications of the scanning and scanning transmission electron microscope part 5 advances in biomedical applications of the scanning electron microscope part 6 scanning electron microscopy in reproductive biology part 7 plant sciences applications of the scanning electron microscope part 8 zoological applications of the scanning electron microscope toronto ontario canada april 5 9 1976. Scanning Electron Microscopy 708, 1976

Single-particle-ICP-MS advances. Analytical and Bioanalytical Chemistry 408(19): 5051-5052, 2016

A high speed short resolving time coincidence circuit for use with scintillation counters. Review of Scientific Instruments 21(10): 883-883, 1950