+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

A new derivatization strategy for the analysis of phosphopeptides by precursor ion scanning in positive ion mode



A new derivatization strategy for the analysis of phosphopeptides by precursor ion scanning in positive ion mode



Journal of the American Society for Mass Spectrometry 13(8): 996-1003



Although numerous strategies have been devised to analyze protein phosphorylation, an abundant intracellular protein modification, there is still a need for different methods for the analysis of this modification. A method to both detect and localize the phosphorylation within a protein/peptide is especially required. In this paper, a new strategy is described, which makes use of beta-elimination/Michael addition reactions to introduce a functional group at the original site of phosphorylation, which gives rise to a dimethylamine-containing sulfenic acid derivative with a unique m/z value. This enables the detection of the phosphorylated species within peptide mixtures by sensitive and specific precursor ion scanning in positive ion mode. Working under acidic conditions in positive ion mode has the added advantage that subsequent normal peptide sequencing for the exact localization can be performed. No other peptide derived fragment ion is observed at the m/z value of the sulfenic acid derivative formed, thus specific precursor ion experiments can also be carried out on instruments with low fragment ion resolution and lends itself to LC-MS/MS approaches when skimmer fragmentation routines or triple quadrupole mass spectrometers are used.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 045079845

Download citation: RISBibTeXText

PMID: 12216740

DOI: 10.1016/S1044-0305(02)00415-4


Related references

Detection of tyrosine phosphorylated peptides by precursor ion scanning quadrupole TOF mass spectrometry in positive ion mode. Analytical Chemistry 73(7): 1440-1448, 2001

Detection of arginine dimethylated peptides by parallel precursor ion scanning mass spectrometry in positive ion mode. Analytical Chemistry 75(13): 3107-3114, 2003

Mass spectrometry analysis of phosphopeptides after peptide carboxy group derivatization. Analytical Chemistry 80(21): 8324-8328, 2008

Precursor ion scanning MS identification of cysteine-containing peptides after their selective conversion to phosphopeptides A method to study sulfhydryl redox chemistry in proteins. Molecular & Cellular Proteomics 2(7 Supplement): S50, July, 2003

An innovative strategy for sulfopeptides analysis using MALDI-TOF MS reflectron positive ion mode. Proteomics 12(14): 2247-2257, 2013

Carboxy group derivatization for enhanced electron-transfer dissociation mass spectrometric analysis of phosphopeptides. Proteomics 9(16): 4093-4097, 2009

Identification of phosphorylation sites in phosphopeptides by positive and negative mode electrospray ionization-tandem mass spectrometry. Journal of the American Society for Mass Spectrometry 7(3): 243-249, 1996

Quadrupole time-of-flight versus triple-quadrupole mass spectrometry for the determination of phosphopeptides by precursor ion scanning. Journal of Mass Spectrometry 36(7): 782-790, 2001

A general precursor ion-like scanning mode on quadrupole-TOF instruments compatible with chromatographic separation. Proteomics 6(1): 41-53, 2005

1,2-Dimethylimidazole-4-sulfonyl chloride (DMISC), a novel derivatization strategy for the analysis of propofol by LC-ESI-MS/MS. Analytical and Bioanalytical Chemistry (): -, 2016

LC-MS(n) analysis of isomeric chondroitin sulfate oligosaccharides using a chemical derivatization strategy. Journal of the American Society for Mass Spectrometry 22(9): 1577-1587, 2011

Derivatization of phosphopeptides with mercapto- and amino-functionalized conjugate groups by phosphate elimination and subsequent Michael addition. Organic & Biomolecular Chemistry 3(16): 3039-3044, 2005

HPLC-MRM relative quantification analysis of fatty acids based on a novel derivatization strategy. Analyst 139(23): 6154-6159, 2015

Manipulating the fragmentation patterns of phosphopeptides via gas-phase boron derivatization: determining phosphorylation sites in peptides with multiple serines. Journal of the American Society for Mass Spectrometry 16(12): 1905-1914, 2005

Monitoring of conductivity changes in passive layers by scanning electrochemical microscopy in feedback mode: localization of pitting precursor sites on surfaces of multimetallic phase materials. Analytical Chemistry 79(11): 3996-4005, 2007