+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

A retroviral peptide encoded by mutated env p15E gene is recognized by specific CD8+ T lymphocytes on drug-treated murine mastocytoma P815



A retroviral peptide encoded by mutated env p15E gene is recognized by specific CD8+ T lymphocytes on drug-treated murine mastocytoma P815



International Journal of Immunopharmacology 18(10): 563-576



Highly immunogenic ("xenogenized") tumour variants appear after treatment of murine mastocytoma P815 with the triazene derivative DTIC, a phenomenon associated with the appearance of structurally abnormal p15E env proteins in the variant cells. In the present study, we have isolated and sequenced several p15E cDNA gene fragments amplified by means of polymerase chain reaction (PCR) from parental (P815) and xenogenized (P815/DTIC) tumour cells. Compared to known p15E sequences in parental cells, one p15E sequence from xenogenized cells presented three distinct nucleotide changes, one of which was apparently unique to P815/DTIC DNA and cDNA upon single-nucleotide primer extension assay. One major histocompatibility complex (MHC) class I-binding peptide, corresponding to a putative mutation in the p15E sequence, was tested in parallel with the parental peptide for recognition by P815/DTIC-specific cytotoxic T cells in vitro. The results suggested that the amino acid substitution at the relevant position of the p15E protein may produce an antigenic T cell epitope. By skin test assay of mice primed with either the synthetic peptide or P815/DTIC cells, evidence was obtained that the mutated peptide is immunogenic in vivo, and that the neoepitope is expressed by P815/DTIC cells. In accordance with previous data in the L5178Y/DTIC tumour model system, these findings reinforce the notion that xenogenization of tumour cells may result in the expression of class I-binding mutated peptides of retroviral origin.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 045106481

Download citation: RISBibTeXText

PMID: 9080250

DOI: 10.1016/s0192-0561(96)00065-3


Related references

Immunogenic properties of retroviral protein P15E from drug-treated murine mastocytoma P815. International Journal Of Cancer. 55(2): 344-350, 1993

The peptide recognized by HLA-A68.2-restricted, squamous cell carcinoma of the lung-specific cytotoxic T lymphocytes is derived from a mutated elongation factor 2 gene. Cancer Research 58(22): 5144-5150, 1998

Identification of a second major tumor-specific antigen recognized by CTLs on mouse mastocytoma P815. Journal of Immunology 162(6): 3534-3540, 1999

Anti-estrogens potentiate the immunotherapy of the P815 murine mastocytoma by cytotoxic T lymphocytes. Anticancer Research 17(2a): 1083-1088, 1997

Humoral mechanisms of in vitro inhibition of murine lymphocytes immunoreactivity by mastocytoma cell line p815. Byulleten' Eksperimental'noi Biologii i Meditsiny 105(6): 701-703, 1988

A synthetic peptide derived from a retroviral p15e sequence displays biological activities of p15e and alpha interferon. Journal of Cellular Biochemistry Suppl. (13 Part C): 39, 1989

A single retroviral gag precursor signal peptide recognized by FBL-3 tumor-specific cytotoxic T lymphocytes. Journal of Virology 69(11): 6735-6741, 1995

Aged murine killer T-cell clones acquire specific cytotoxicity for P815 mastocytoma cells. Nature 308(5957): 367-370, 1984

A peptide recognized by human cytolytic T lymphocytes on HLA-A2 melanomas is encoded by an intron sequence of the N-acetylglucosaminyltransferase V gene. Journal of Experimental Medicine 183(3): 1173-1183, 1996

A mutated beta-catenin gene encodes a melanoma-specific antigen recognized by tumor infiltrating lymphocytes. Journal of Experimental Medicine 183(3): 1185-1192, 1996

The influence of synthetic peptide from retroviral transmembrane protein p15E on murine spleen cell proliferation and bone marrow hemopoietic precursor colony formation. Biomedicine & Pharmacotherapy 47(9): 397-402, 1993

Retroviral-mediated gene transfer of murine IL-12 into P815 cells abrogates tumorigenicity. British Journal of Haematology 93(Suppl. 2): 335, 1996

Mutated proteins encoded by a lentivirus mutated env gene, peptide fragments and expression vectors. Official Gazette of the United States Patent & Trademark Office Patents 1228(5), 1999