+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Absence of continuous epitopes in the house dust mite major allergens Der p I from Dermatophagoides pteronyssinus and Der f I from Dermatophagoides farinae



Absence of continuous epitopes in the house dust mite major allergens Der p I from Dermatophagoides pteronyssinus and Der f I from Dermatophagoides farinae



Clinical and Experimental Allergy 26(1): 36-42



The house dust mite has been shown to be an important source of domestic allergens associated with immediate hypersensitivities. The Group I mite allergens Der p I from Dermatophagoides pteronyssinus and Der f I from D. farinae display extensive amino acid sequence homology and have similarities with cysteine protease enzymes. The availability of the complete amino acid sequences for these allergens allowed us to search for the allergic determinants within these molecules. The aim of the present investigation was to identify any continuous IgE-binding epitopes within these amino acid sequences. We also sought to test the validity of previously reported Der p I peptide epitope sequences. In order to identify any continuous IgE epitopes, the amino acid sequences of Der p I and Der f I were synthesized as decapeptides overlapping in sequence and coupled to plastic pins. The specific IgE-binding capacity of these peptides was assayed using an enzyme-linked biotin-streptavidin procedure and sera from patients known to be sensitive to these allergens. Previously reported Der p I peptide epitopes were synthesized as free peptides and tested for their ability to inhibit specific IgE binding to allergen extract discs. None of the pin-coupled Der p I or Der f I peptides was found by the continuous epitope mapping procedure to bind significantly to specific IgE in the sera of hypersensitive patients. The previously reported Der p I peptide epitopes did not inhibit specific IgE binding to mite extract discs. The specific IgE binding epitopes of the house dust mite allergens Der p I and Der f I are discontinuous in nature.

(PDF emailed within 0-6 h: $19.90)

Accession: 045141832

Download citation: RISBibTeXText

PMID: 8789541

DOI: 10.1111/j.1365-2222.1996.tb00054.x


Related references

Elisa for determination of major excrement allergens of house dust mite species dermatophagoides pteronyssinus dermatophagoides farinae and dermatophagoides microceras. Allergy 41(6): 442-451, 1986

Major house dust mite allergens Dermatophagoides pteronyssinus 1 and Dermatophagoides farinae 1 degrade and inactivate lung surfactant proteins A and D. Journal of Biological Chemistry 282(51): 36808-36819, 2007

Demonstration of close physicochemical similarity and partial immunochemical identity between the major allergen dp 42 of the house dust mite dermatophagoides pteronyssinus and corresponding antigens of dermatophagoides farinae df 6 and dermatophagoides microceras dm 6. International Archives of Allergy & Applied Immunology 79(1): 60-65, 1985

Immune reactivity of recombinant group 2 allergens of house dust mite, Dermatophagoides pteronyssinus, and Dermatophagoides farinae. Journal of Investigational Allergology & Clinical Immunology 13(1): 36-42, 2003

Identification of major allergens from the house dust mites, Dermatophagoides farinae and Dermatophagoides pteronyssinus, by electroblotting. Yonsei Medical Journal 32(1): 24-32, 1991

Cross reacting and species specific determinants on a major allergen from dermatophagoides pteronyssinus and dermatophagoides farinae development of a radioimmunoassay for antigen p 1 equivalent in house dust and dust mite extracts. Journal of Allergy & Clinical Immunology 78(3 PART 1): 398-407, 1986

Reactivity to intradermal injection of extracts of Dermatophagoides farinae, Dermatophagoides pteronyssinus, house dust mite mix, and house dust in dogs suspected to have atopic dermatitis: 115 cases (1996-1998). Journal of the American Veterinary Medical Association 217(4): 536-540, 2000

Mites and house dust allergy. II. Relationship between house dust and mite (Dermatophagoides pteronyssinus and D. farinae) allergens by fractionation methods. Clinical Allergy 2(2): 115-123, 1972

Cross reacting and species specific epitopes on major allergens from dermatophagoides pteronyssinus and dermatophagoides farinae defined with monoclonal antibodies. Journal Of Allergy & Clinical Immunologypart 2: 146, 1985

House dust mite (Dermatophagoides farinae and Dermatophagoides pteronyssinus) prevalence in the rooms and hallways of a tertiary care hospital. Journal of Allergy and Clinical Immunology 95(4): 801-805, 1995

The stability of aqueous and glycerinated house dust mite extracts dermatophagoides pteronyssinus and dermatophagoides farinae as measured by rast inhibition. Journal of Allergy & Clinical Immunology 85(1 PART 2): 238, 1990

Intradermal skin testing with Dermatophagoides pteronyssinus and Dermatophagoides farinae in a southern United States area where DP is the predominate house dust mite. Journal of Allergy & Clinical Immunology 99(1 PART 2): S346, 1997

Enzymatic analyses of house dust mite extracts from Dermatophagoides pteronyssinus and Dermatophagoides farinae (Acari: Pyroglyphidae) during different phases of culture growth. Journal of Medical Entomology 36(3): 370-375, 1999

The early changes of Dermatophagoides farinae -specific IgE, IgG1, and IgG4 after rush immunotherapy with Df and Dermatophagoides pteronyssinus in house dust mite sensitive asthma. Journal of Allergy & Clinical Immunology 107(2): S255, 2001

Purification and partial characterization of two major allergens from the house dust mite Dermatophagoides pteronyssinus. Journal of Allergy and Clinical Immunology 76(5): 753-761, 1985