+ Translate
+ Most Popular
The pigeon tick (Argas reflexus): its biology, ecology, and epidemiological aspects
Prevalence of hemoglobin abnormalities in Kebili (Tunisian South)
Lipogranuloma: a preventable complication of dacryocystorhinostomy
Value of basal plasma cortisol assays in the assessment of pituitary-adrenal insufficiency
Bees from the Belgian Congo. The acraensis group of Anthophora
Placing gingival retraction cord
Total serum IgE, allergy skin testing, and the radioallergosorbent test for the diagnosis of allergy in asthmatic children
Acariens plumicoles Analgesoidea parasites des oiseaux du Maroc
Injuries of terminal phalanges of the fingers in children
Biology of flowering and nectar production in pear (Pyrus communis)
Das Reliktvorkommen der Aspisviper (Vipera aspis L.) im Schwarzwald
Hydrological modelling of drained blanket peatland
Pathologic morphology and clinical significance of the anomalous origin of the left circumflex coronary artery from the right coronary artery. General review and autopsy analysis of 30 cases
Cyto genetic analyses of lymphocyte cultures after exposure to calcium cyclamate
Axelrodia riesei, a new characoid fish from Upper Rio Meta in Colombia With remarks concerning the genus Axelrodia and description of a similar, sympatric, Hyphessobrycon-species
Favorable evolution of a case of tuberculosis of pancreas under antibiotic action
RIFM fragrance ingredient safety assessment, Valencene, CAS Registry Number 4630-07-3
Parenteral microemulsions: an overview
Temperate pasture: management for grazing and conservation
Evaluation of a new coprocessed compound based on lactose and maize starch for tablet formulation
Thermal expansion and cracking of three confined water-saturated igneous rocks to 800C
Revision of the genera of the tribe Stigmoderini (Coleoptera: Buprestidae) a discussion of phylogenetic relationships
Anal tuberculosis. Report of a case
Gastric tuberculosis in the past and present
Adaptive responses of the cardiovascular system to prolonged spaceflight conditions: assessment with Holter monitoring

Activation of K+/Cl- cotransport in human erythrocytes exposed to oxidative agents

Activation of K+/Cl- cotransport in human erythrocytes exposed to oxidative agents

Biochimica et Biophysica Acta 1176(1-2): 37-42

ISSN/ISBN: 0006-3002

PMID: 8452877

DOI: 10.1016/0167-4889(93)90174-n

Activation of K+/Cl- cotransport was studied after exposure of normal human erythrocytes to the oxidative action of acetylphenylhydrazine (APH), menadione sodium bisulfite (MSB), hydrogen peroxide (H2O2) or phenazine metasulfate (PMS). In order to better define the relative contributions of K+/Cl- cotransport on ouabain and bumetanide-resistant (OBR) K+ efflux induced by oxidation, we used (dihydroindenyl)oxyalkanoic acid (DIOA) and carbocyanine as specific inhibitors, respectively, of cotransport system and Ca(2+)-activated K+ channel. APH, MSB and - to much less extent - H2O2 promoted a K+ efflux pathway with features corresponding to those of K+/Cl- cotransport. This pathway showed: (i) kinetics of efflux compatible with a specific cation transport system; (ii) requirement for chloride anion; (iii) resistance to ouabain, bumetanide and carbocyanine inhibition; (iv) stimulation by hypotonic challenge; (v) susceptibility to inhibition by DIOA. Dithiothreitol (DTT) or 2-mercaptoethanol (2-ME) decreased K+/Cl- cotransport activation, suggesting that oxidative mechanisms affected crucial SH groups of the transporter. These data suggest that oxidation represents a factor capable of modulating activation of K+/Cl- cotransport. Its possible contribution in situations with high oxidative risk, such as sickle-cell anaemia or beta thalassemia, is discussed.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 045156423

Download citation: RISBibTeXText

Related references

Membrane transport of Na and K in thalassemic erythrocytes Role of oxidative damage in K-Cl cotransport activation. Blood 82(10 Suppl. 1): 361A, 1993

Oxidative activation of K-Cl cotransport by diamide in erythrocytes from humans with red cell disorders, and from several other mammalian species. Journal of Membrane Biology 155(3): 207-217, 1997

Role of protein phosphatase in activation of KCl cotransport in human erythrocytes. American Journal of Physiology 260(1 Pt 1): C176-C180, 1991

KCl cotransport activation in human erythrocytes by high hydrostatic pressure. Journal of Physiology 491: 423-434, 1996

Role of protein phosphatase in activation of potassium chloride cotransport in human erythrocytes. American Journal of Physiology 260(1 Part 1): C176-C180, 1991

Activation of K-Cl cotransport by the oxidant diamide in density-separated human and sheep erythrocytes. FASEB Journal 10(3): A665, 1996

Copper-specific damage in human erythrocytes exposed to oxidative stress. Biological Trace Element Research 56(2): 231-240, 1997

Verapamil prevents impairment in filterability of human erythrocytes exposed to oxidative stress. Japanese Journal of Physiology 54(1): 39-46, 2004

Oxidative stress biomarkers and acetylcholinesterase activity in human erythrocytes exposed to clomazone (in vitro). Interdisciplinary Toxicology 4(3): 149-153, 2011

Activation of Na+/H+ exchange and Na+,K+,2Cl(-)-cotransport in rat erythrocytes during hyperosmotic shrinkage. Biologicheskie Membrany 22(2): 98-104, 2005

Variations of adenine nucleotide levels in normal and pathologic human erythrocytes exposed to oxidative stress. Italian Journal of Biochemistry 38(6): 404A-406A, 1989

Proteolysis in human erythrocytes is triggered only by selected oxidative stressing agents. Italian Journal of Biochemistry 37(3): 129-138, 1988

Swelling activation of K-Cl cotransport in LK sheep erythrocytes: a three-state process. Journal of General Physiology 101(5): 733-765, 1993

Dissociation of hypertension and abnormal activation of potassium chloride cotransport in shr erythrocytes. Sassard, J (Ed ) Colloques Inserm (Institut National De La Sante Et De La Recherche Medical), Vol 218 Hypertension Genetique; (Inserm (National Institute Of Health And Medical Research) Colloquium, Vol 218 Genetic Hypertension); Seventh International Symposium on Shr (Spontaneously Hypertensive Rats) And Related Studies, Lyon, France, October 28-30, 1991 Xxii+618p Editions John Libbey Eurotext: Montrouge, France; London, England, Uk; Editions Inserm: Paris, France Illus Paper 33, 1992

Kinetics and peculiarities of thermal inactivation of volume-induced Na+/H+ exchange, Na+,K+,2Cl- cotransport and K+,Cl- cotransport in rat erythrocytes. Biochimica et Biophysica Acta 1151(2): 186-192, 1993