Activity of xylose reductase from Candida mogii grown in media containing different concentrations of rice straw hydrolysate
Mayerhoff, Z.D.; Roberto, I.C.; Franco, T.T.
Applied Biochemistry and Biotechnology 91-93: 729-737
2001
ISSN/ISBN: 0273-2289 PMID: 11963901 DOI: 10.1385/abab:91-93:1-9:729
Accession: 045160205
Xylose reductase (XR) activity was evaluated in extracts of Candida mogii grown in media containing different concentrations of rice straw hydrolysate. Results of XR activity were compared to xylitol production and a similar behavior was observed for these parameters. Highest values of specific production and productivity were found for xylose reductase (35 U/g of cell and 0.97 U/[g of cell x h], respectively) and for xylitol (5.63 g/g of cell and 0.13 g/[g of cell x h]) in fermentation conducted in medium containing 49.2 g of xylose/L. The maximum value of XR:XD ratio (1.82) was also calculated under this initial xylose concentration with 60 h of fermentation.