+ Translate
+ Most Popular
The pigeon tick (Argas reflexus): its biology, ecology, and epidemiological aspects
Prevalence of hemoglobin abnormalities in Kebili (Tunisian South)
Lipogranuloma: a preventable complication of dacryocystorhinostomy
Value of basal plasma cortisol assays in the assessment of pituitary-adrenal insufficiency
Bees from the Belgian Congo. The acraensis group of Anthophora
Placing gingival retraction cord
Total serum IgE, allergy skin testing, and the radioallergosorbent test for the diagnosis of allergy in asthmatic children
Acariens plumicoles Analgesoidea parasites des oiseaux du Maroc
Injuries of terminal phalanges of the fingers in children
Biology of flowering and nectar production in pear (Pyrus communis)
Das Reliktvorkommen der Aspisviper (Vipera aspis L.) im Schwarzwald
Hydrological modelling of drained blanket peatland
Pathologic morphology and clinical significance of the anomalous origin of the left circumflex coronary artery from the right coronary artery. General review and autopsy analysis of 30 cases
Cyto genetic analyses of lymphocyte cultures after exposure to calcium cyclamate
Axelrodia riesei, a new characoid fish from Upper Rio Meta in Colombia With remarks concerning the genus Axelrodia and description of a similar, sympatric, Hyphessobrycon-species
Favorable evolution of a case of tuberculosis of pancreas under antibiotic action
RIFM fragrance ingredient safety assessment, Valencene, CAS Registry Number 4630-07-3
Parenteral microemulsions: an overview
Temperate pasture: management for grazing and conservation
Evaluation of a new coprocessed compound based on lactose and maize starch for tablet formulation
Thermal expansion and cracking of three confined water-saturated igneous rocks to 800C
Revision of the genera of the tribe Stigmoderini (Coleoptera: Buprestidae) a discussion of phylogenetic relationships
Anal tuberculosis. Report of a case
Gastric tuberculosis in the past and present
Adaptive responses of the cardiovascular system to prolonged spaceflight conditions: assessment with Holter monitoring

Adaptive responses of Ralstonia eutropha to feast and famine conditions analysed by flow cytometry

Adaptive responses of Ralstonia eutropha to feast and famine conditions analysed by flow cytometry

Journal of Biotechnology 75(2-3): 81-97

ISSN/ISBN: 0168-1656

PMID: 10617338

Results obtained by flow cytometry allow conclusions to be drawn about how the physiological states of Ralstsonia eutropha JMP134 are connected with survival strategies under distinct growth conditions. During both feast and famine conditions the cells were found to proceed through sharply separated phases of life. Two sources of carbon and energy, one poor (0.02% phenol) and one rich (0.2% pyruvate and 0.1% yeast extract) were chosen to study the cellular responses. Despite the major differences in carbon source, when growth stages of the bacteria on the two substrates were characterised in batch growth, only minor differences were found in the time course of the membrane potential related fluorescence intensity (MPRFI). This also applied to the rRNA content and the size-correlated forward scatter (FSC) signal of the cells, both of which increased to high levels during the (early) exponential growth phase. On the rich medium, DNA synthesis initially occurred in an uncoupled manner, then a high rate of PHB formation followed when nutrients began to be limiting. Under famine conditions, the cellular responses were much more complex. PHB was synthesised, then DNA synthesis occurred in a 'eukaryotic' mode, to be succeeded by renewed PHB synthesis. To obtain defined cell physiological states, the chemostat technique was used in addition to batch experiments. The results obtained clearly indicated that key events in cell physiology, including initiation of DNA replication and overflow metabolism, occurred in a hierarchically ordered manner and were tightly correlated with changes in the environmental conditions of the bacterial cells.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 045170728

Download citation: RISBibTeXText

Related references

Adaptive responses of Ralstonia eutropha to feast and famine conditions analysed by flow cytometry. Journal of Biotechnology 75(2-3): 81-97, 1999

Adaptive responses to static conditions in nutrient-rich cultures of luminous Ralstonia eutropha. Biotechnology Letters 26(7): 559-562, 2004

The adaptive responses of Escherichia coli to a feast and famine existence. Advances in Microbial Physiology 6: 147-217, 1971

Dynamic response of aerobic granular sludge to feast and famine conditions in plug flow reactors fed with real domestic wastewater. Science of the Total Environment 758: 144155, 2020

Transfer of two Burkholderia and an Alcaligenes species to Ralstonia gen. Nov.: Proposal of Ralstonia pickettii (Ralston, Palleroni and Doudoroff 1973) comb. Nov., Ralstonia solanacearum (Smith 1896) comb. Nov. and Ralstonia eutropha (Davis 1969) comb. Nov. Microbiology and Immunology 39(11): 897-904, 1995

Beyond feast and famine: Selecting a PHA accumulating photosynthetic mixed culture in a permanent feast regime. Water Research 105: 421-428, 2016

Effects of periodic feast/famine conditions on the growth of biofilms. Environmental Technology Letters 8(1-12): 87-94, 1987

Feast/famine ratio determined continuous flow aerobic granulation. Science of the Total Environment 750: 141467, 2021

Modeling microbial products in activated sludge under feast-famine conditions. Environmental Science and Technology 43(7): 2489-2497, 2009

Feast and famine: critical role of glucocorticoids with insulin in daily energy flow. Frontiers in Neuroendocrinology 14(4): 303-347, 1993

Community Assembly and Ecology of Activated Sludge under Photosynthetic Feast-Famine Conditions. Environmental Science and Technology 51(6): 3165-3175, 2017

Proteomic examination of Ralstonia eutropha in cellular responses to formic acid. Proteomics 6(15): 4259-4268, 2006

In situ identification of polyhydroxyalkanoate (PHA)-accumulating microorganisms in mixed microbial cultures under feast/famine conditions. Scientific Reports 10(1): 3752, 2020

Native habitat mitigates feast-famine conditions faced by honey bees in an agricultural landscape. Proceedings of the National Academy of Sciences of the United States of America 116(50): 25147-25155, 2019

Evidence that Ralstonia eutropha (Alcaligenes eutrophus) contains a functional homologue of the Ralstonia solanacearum Phc cell density sensing system. Molecular Microbiology 38(2): 359-367, 2000