+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Adhesion of cerebral endothelial cells to lymphocytes from patients with multiple sclerosis

Adhesion of cerebral endothelial cells to lymphocytes from patients with multiple sclerosis

Autoimmunity 14(4): 329-333

To investigate the factors regulating the entry of lymphocytes into the brain, we assessed the adhesion in vitro of 51Cr labelled lymphocytes from peripheral blood of patients with multiple sclerosis (MS) to human cerebral endothelial cells, and evaluated the effect on the adhesion of endothelium activated by interferon-gamma (IFN-gamma), lipopolysaccharides (LPS), interleukin-1 (IL-1) and tumor necrosis factor (TNF). Patients with acute relapsing MS during an exacerbation showed significant increase in MNC adherence to cerebral endothelial cells as compared with controls (p < 0.001). MNC adherence to cerebral endothelial cells activated by IFN-gamma or LPS, was significantly increased as compared to the controls (p < 0.01). MNC adherence to endothelial cells was not blocked by antibodies against the intercellular adhesion molecule-1 (ICAM-1), but was blocked by lymphocyte function-associated antigen-1 (LFA-1). The increased adherence observed in patients with acute relapsing MS during an exacerbation would modulate the migration of lymphocytes across the blood-brain barrier (BBB).

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 045174923

Download citation: RISBibTeXText

PMID: 8347775

Related references

Brain microvascular endothelial cells and leukocytes derived from patients with multiple sclerosis exhibit increased adhesion capacity. Neuroreport 8(3): 629-633, 1997

Adhesion of mononuclear cells from multiple sclerosis patients to cerebral vessels in cryostat sections of normal human brain. European Journal of Neurology 6(5): 579-584, 1999

Enhanced binding of lymphocytes from patients with multiple sclerosis to tumour necrosis factor-alpha (TNF-alpha)-treated endothelial monolayers: associations with clinical relapse and adhesion molecule expression. Clinical and Experimental Immunology 105(1): 155-162, 1996

Destruction of nerve cells cultured "in vitro" by lymphocytes from patients with multiple sclerosis. Auto-immune pathogenesis of multiple sclerosis. La Presse Medicale 77(55): 2103-2106, 1969

Increased adhesion of CD4+ and CD14+ mononuclear cells to cultured multiple sclerosis brain endothelial cells. Journal Of Neurology. 243(9): 666-667, 1996

Lymphocytes subgroups and expression of endothelial cell adhesion molecules in the skin of patients with systemic sclerosis. Journal of Investigative Dermatology 94(4): 573, 1990

IgM antiendothelial cell antibodies from patients with Behcets disease can induce the expression of adhesion molecules and the adhesion of T lymphocytes to endothelial cells. Journal of Investigative Dermatology 112(4): 648, 1999

In vitro IgG synthesis by peripheral blood lymphocytes of multiple sclerosis patients and normal individuals. Effect of multiple sclerosis and non-multiple sclerosis brain antigens. European Neurology 22(2): 131-137, 1983

In vitro intercellular adhesion molecule-1 expression on brain endothelial cells in multiple sclerosis. Journal Of Neuroimmunology. 49(1-2): 181-187, 1994

Increased adhesion of mononuclear cells to cerebral vascular endothelium in multiple sclerosis. Brain Pathology 7(4): 1361, 1997

Adhesion molecules on endothelial cells in the central nervous system: an emerging area in the neuroimmunology of multiple sclerosis. Clinical Immunology and Immunopathology 57(2): 173-187, 1990

Endothelial cell activation in cerebral microvessels from patients with multiple sclerosis. Neurology 42(4 Suppl. 3): 403, 1992

Expression pattern of activation and adhesion molecules on peripheral blood CD4+ T-lymphocytes in relapsing-remitting multiple sclerosis patients: a serial analysis. Journal of Neuroimmunology 66(1-2): 147-151, 1996

Expression of adhesion molecules on the CSF and blood lymphocytes and macrophages in patients with secondary progressive multiple sclerosis Correlation to neurological dysfunction and MS-related MRI changes. Neurology 50(4 Suppl. 4): A109-A110, 1998

Proteomic analysis of human cerebral endothelial cells activated by multiple sclerosis serum and IFNbeta-1b. Journal of Molecular Neuroscience 32(3): 169-178, 2007