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Alpha 1-adrenergic agonist-stimulated protein secretion in rat exorbital lacrimal gland acini

Alpha 1-adrenergic agonist-stimulated protein secretion in rat exorbital lacrimal gland acini

Experimental Eye Research 58(4): 423-429

The alpha-adrenergic stimulation by phenylephrine of lacrimal gland protein secretion was pharmacologically characterized. Acini, prepared from rat exorbital lacrimal glands, were incubated with agonists, antagonists or both for 0-20 min. Peroxidase secretion, an index of protein secretion, was measured spectrophotometrically. Peroxidase secretion was stimulated by the alpha 1-adrenergic agonists clonidine. The non-selective alpha-adrenergic antagonist phentolamine completely inhibited phenylephrine-induced secretion. The selective alpha 1-adrenergic alkylating agent phenoxybenzamine and the selective alpha 1-adrenergic antagonist prazosin partially inhibited phenylephrine-induced secretion. The alpha 2-adrenergic antagonist yohimbine, the beta-adrenergic antagonist timolol, and the dopaminergic antagonist haloperidol also inhibited phenylephrine-induced secretion but were 100-fold less potent than prazosin. It is concluded that phenylephrine activates an alpha 1-adrenergic pathway, but not an alpha 2-adrenergic, beta-adrenergic or dopaminergic pathway, to stimulate lacrimal gland protein secretion from acini.

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Accession: 045203761

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PMID: 7925679

DOI: 10.1006/exer.1994.1035

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