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Application of long-distance PCR to restriction site mapping of a cloned DNA fragment on the lambda EMBL3 phage vector

Machida, M.; Manabe, M.; Yasukawa, M.; Jigami, Y.

Bioscience Biotechnology and Biochemistry 60(6): 1011-1013

1996

ISSN/ISBN: 0916-8451
PMID: 8695900
DOI: 10.1271/bbb.60.1011
Accession: 045290706
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Long-distance PCR was applied to rapidly map the restriction sites of long inserts cloned on lambda EMBL3 phage vector. The restriction sites of 9 of 15 enzymes were completely assigned in a model experiment within 14 h, including 8h for the PCR amplification. This method was found particularly useful for genomic DNA cloning when the partial sequence of the corresponding cDNA is known.

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Related References

Machida, M.; Manabe, M.; Yasukawa, M. 1996: Application of long-distance PCR to restriction site mapping of a cloned DNA fragment on the lEMBL3 phage vector Bioscience, Biotechnology, and Biochemistry 60: 11-13
Machida, M.; Manabe, M.; Yasukawa, M.; Jigami, Y. 1996: Application of long-distance PCR to restriction site mapping of a cloned DNA fragment on the λEMBL3 phage vector Bioscience, Biotechnology, and Biochemistry 60(6): 1011-1013
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Blumenberg, M. 1978: Mapping the restriction sites in bacterio phage lambda dna Catsimpoolas, Nicholas (Ed ) Developments in Biochemistry, Vol 2 Electrophoresis '78 Proceedings Of The International Conference Cambridge, Mass , Usa, Apr 19-21, Xi+443p Illus Elsevier North Holland, Inc: New York, N Y , Usa (Dist By Elsevier/North-Holland Biomedical Press: Amsterdam, The Netherlands ) ISBN 0-44-00294-4: 325-336
Grimm, S.; Pflugfelder, G.O. 1995: Nonradioactive method for restriction mapping of lambda phage DNA Biotechniques 18(3): 400-401
Luk, K.C.; Szybalski, W. 1982: Characterization of the cloned terminators tR1, tL3 and tI and the nut R antitermination site of coliphage lambda Gene 20(2): 127-134
Frischauf, A.M.; Murray, N.; Lehrach, H. 1987: Lambda phage vectors--EMBL series Methods in Enzymology 153: 103-115
Ovchinnikov, Y.A.; Guryev, S.O.; Krayev, A.S.; Monastyrskaya, G.S.; Skryabin, K.G.; Sverdlov, E.D.; Zakharyev, V.M.; Bayev, A.A. 1979: Primary structure of an eco r i fragment of phage lambda imm 434 dna containing regions ci cro of phage 434 and cii o of phage lambda Gene 6(3): 235-250
Piñol, J.; Cabré, O. 1994: Restriction mapping of phage lambda vectors using non-radioactive methods Gene 143(1): 39-41
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