EurekaMag
+ Translate
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Beta1 integrin cytoplasmic domain regulates the constitutive conformation detected by MAb 15/7, but not the ligand-induced conformation


Beta1 integrin cytoplasmic domain regulates the constitutive conformation detected by MAb 15/7, but not the ligand-induced conformation



Journal of Cellular Biochemistry 71(1): 63-73



ISSN/ISBN: 0730-2312

PMID: 9736455

DOI: 10.1002/(sici)1097-4644(19981001)71:1<63::aid-jcb7>3.0.co;2-#

The anti-integrin beta1 MAb 15/7 sometimes may be a reporter of integrin activation or ligand occupancy. However, certain beta1 tail deletions eliminate ligand binding despite inducing maximal constitutive 15/7 expression [Puzon-Mclaughlin et al. (1 996): J Biol Chem 271:16580-16585]. Here we describe beta1 tail mutations (e.g., double point mutations [D759L/F763L, F766L/E769L], or replacement of the beta1 tail by the beta5 tail) that prevent rather than induce constitutive appearance of the 15/7 epitope. Despite variable losses of constitutive 15/7 epitope, these mutants all retained a similar inducible 15/7 epitope component as seen upon incubation with GRGDSP peptide ligand. In addition, constitutive 15/7 expression did not correlate with integrin localization into focal adhesions. In conclusion, we show for the first time for a fully functional integrin that specific mutations within the beta1 tail can down-regulate the constitutive appearance of an extracellular conformation defined by MAb 15/7. Because this regulation occurs away from the ligand binding site and does not correlate with responsiveness to integrin ligand, cell adhesion, or localization into focal adhesions, a novel type of conformational regulation is suggested.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 045363764

Download citation: RISBibTeXText

Related references

Regulation of conformation and ligand binding function of integrin a5b1 by the b1 cytoplasmic domain. The Journal of Biological Chemistry 271: 580-5, 1996

Regulation of conformation and ligand binding function of integrin alpha5beta1 by the beta1 cytoplasmic domain. Journal of Biological Chemistry 271(28): 16580-16585, 1996

Identification of a ligand binding domain of beta-2 integrin which alters a divalent cation-dependent conformation. Molecular Biology of the Cell 6(Suppl ): 284A, 1995

A beta turn in the cytoplasmic tail of the integrin alpha v subunit influences conformation and ligand binding of alpha v beta 3. Journal of Biological Chemistry 269(6): 4641-4647, 1994

A beta turn in the alpha-V subunit cytoplasmic tail influences the conformation and ligand binding properties of integrin alpha-V-beta-3. Molecular Biology of the Cell 4(Suppl ): 284A, 1993

Distinct ligand binding sites in the I domain of integrin alpha M beta 2 that differentially affect a divalent cation-dependent conformation. Journal of Biological Chemistry 270(43): 25866-25871, 1995

A Matricryptic Conformation of the Integrin-Binding Domain of Fibronectin Regulates Platelet-Derived Growth Factor-Induced Intracellular Calcium Release. Cells 8(11), 2019

A β turn in the cytoplasmic tail of the integrin αV subunit influences conformation and ligand binding of αVβ3. The Journal of Biological Chemistry 269(6): 4641-4647, 1994

A b turn in the cytoplasmic tail of the integrin av subunit influences conformation and ligand binding of avb3. The Journal of Biological Chemistry 269: 41-7, 1994

A novel pesticide-induced conformational state of the oestrogen receptor ligand-binding domain, detected by conformation-specific peptide binding. Febs Letters 579(2): 541-548, 2005

The Talin head domain binds to integrin beta subunit cytoplasmic tails and regulates integrin activation. Journal of Biological Chemistry 274(40): 28071-28074, 1999

A functional comparison of mutations in integrin beta cytoplasmic domains: effects on the regulation of tyrosine phosphorylation, cell spreading, cell attachment and beta1 integrin conformation. Journal of Cell Science 114(Pt 15): 2795-2807, 2001

Formation of hemidesmosome-like structures in the absence of ligand binding by the (alpha)6(beta)4 integrin requires binding of HD1/plectin to the cytoplasmic domain of the (beta)4 integrin subunit. Journal of Cell Science 113: 963-973, 2000

Distinct ligand binding sites in the I domain of integrin aMb2 that differentially affect a divalent cation-dependent conformation. The Journal of Biological Chemistry 270: 866-71, 1995

A beta 3 integrin mutation abolishes ligand binding and alters divalent cation-dependent conformation. Science 249(4971): 915-918, 1990