+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Bone sialoprotein-induced reparative dentinogenesis in the pulp of rat's molar

Bone sialoprotein-induced reparative dentinogenesis in the pulp of rat's molar

Clinical Oral Investigations 4(2): 110-119

Bone sialoprotein (BSP), an osteogenic protein (OP), mixed with a carrier, was implanted in the pulp of rat first upper molars (OP group). Cavities were prepared with dental burs and pulp perforation was carried out by pressure with the tip of a steel probe. After 8, 14, and 30 days, the rats were killed and the pulps of the OP group were compared with (1) a sham group (S group), (2) a group where the carrier was implanted alone (C group), and (3) capping with calcium hydroxide (Ca group). After 8 days, a few inflammatory cells were seen, mostly located at the pulp surface near the perforation. In the Ca group, a dentin bridge started to form, in contrast to the other groups. After 15 days, globular structures were seen in the pulps of the S and C groups. A reparative osteodentin bridge isolated the pulp from the cavity in the Ca group. Variable reactions were seen in the OP group, with some evidence of cell and matrix alignments or plugs of osteodentin in continuity with an inner layer of reparative dentin. After 30 days, irregular osteodentin formation was observed in the pulps of the S and C groups, with a tendency for globular structures to merge, but with interglobular spaces filled by pulp remnants. In the Ca group, osteodentin was observed in the mesial part of the pulp chamber. In the BSP-implanted group, the osteogenic protein stimulated the formation of a homogeneous dentin-like deposit occupying most of the mesial part of the pulp. Apparently, BSP stimulates the differentiation of cells which secrete an organized extracellular matrix more efficiently than any other capping material used so far. Altogether, the results reported here support that bone sialoprotein displays novel bioactive properties and is capable of stimulating in 1 month's time the development of a thick reparative dentinal tissue in the pulp, occluding the perforation and filling the mesial third of the pulp chamber.

(PDF emailed within 1 workday: $29.90)

Accession: 045394333

Download citation: RISBibTeXText

PMID: 11218498

Related references

Immunohistochemistry of bone sialoprotein and osteopontin during reparative dentinogenesis in vivo. Chinese Journal of Dental Research 3(2): 38-43, 2001

Course or reparative dentinogenesis in the rat molar. Ceskoslovenska Stomatologie 76(2): 89-91, 1976

Rate of reparative dentinogenesis under a pulp-capping agent in monkeys. Journal of Dental Research 56(1): 93-93, 1977

Role of CTGF/CCN2 in reparative dentinogenesis in human dental pulp. Journal of Oral Science 54(1): 47-54, 2012

Immunolocalization of fibronectin during reparative dentinogenesis in human teeth after pulp capping with calcium hydroxide. Journal of Dental Research 75(8): 1590-1597, 1996

Inability of calcium hydroxide to induce reparative dentinogenesis at non-peripheral sites of dog dental pulp. European Journal Of Oral Sciences. 104(5-6): 623-626, 1996

Immunolocalization of bone extracellular matrix proteins (type I collagen, osteonectin and bone sialoprotein) in human dental pulp and cultured pulp cells. International Endodontic Journal 36(6): 404-410, 2003

Human tooth culture: a study model for reparative dentinogenesis and direct pulp capping materials biocompatibility. Journal of Biomedical Materials Research. Part B, Applied Biomaterials 85(1): 180-187, 2007

Immunolocalization of fibronectin during reparative dentinogenesis in rat molor teeth after pulp capping with mineral trioxide aggregate or calcium hydroxide. New York State Dental Journal 77(6): 36-42, 2012

Temporal and spatial expression of c-jun and jun-B proto-oncogenes in pulp cells involved with reparative dentinogenesis after cavity preparation of rat molars. Journal of Dental Research 78(2): 673-680, 1999

P43-implantation of odontoblast progenitors in the rat molar pulp leads to the formation of reparative osteodentin. Bulletin du Groupement International Pour la Recherche Scientifique en Stomatologie & Odontologie 49(3): 109-110, 2013

Osteogenic proteins (bone sialoprotein and bone morphogenetic protein-7) and dental pulp mineralization. Journal of Materials Science. Materials in Medicine 13(2): 225-232, 2004

Reparative dentinogenesis induced by mineral trioxide aggregate: a review from the biological and physicochemical points of view. International Journal of Dentistry 2009: 464280-464280, 2009

Providing an environment for reparative dentine induction in amputated rat molar pulp by high molecular-weight hyaluronic acid. Archives of Oral Biology 40(3): 209-219, 1995

Expression of keratin and bone sialoprotein during murine molar tooth root development. Molecular Biology of the Cell 7(SUPPL ): 621A, 1996