EurekaMag
+ Translate
+ Most Popular
The pigeon tick (Argas reflexus): its biology, ecology, and epidemiological aspects
Prevalence of hemoglobin abnormalities in Kebili (Tunisian South)
Lipogranuloma: a preventable complication of dacryocystorhinostomy
Value of basal plasma cortisol assays in the assessment of pituitary-adrenal insufficiency
Bees from the Belgian Congo. The acraensis group of Anthophora
Placing gingival retraction cord
Total serum IgE, allergy skin testing, and the radioallergosorbent test for the diagnosis of allergy in asthmatic children
Acariens plumicoles Analgesoidea parasites des oiseaux du Maroc
Injuries of terminal phalanges of the fingers in children
Biology of flowering and nectar production in pear (Pyrus communis)
Das Reliktvorkommen der Aspisviper (Vipera aspis L.) im Schwarzwald
Hydrological modelling of drained blanket peatland
Pathologic morphology and clinical significance of the anomalous origin of the left circumflex coronary artery from the right coronary artery. General review and autopsy analysis of 30 cases
Cyto genetic analyses of lymphocyte cultures after exposure to calcium cyclamate
Axelrodia riesei, a new characoid fish from Upper Rio Meta in Colombia With remarks concerning the genus Axelrodia and description of a similar, sympatric, Hyphessobrycon-species
Favorable evolution of a case of tuberculosis of pancreas under antibiotic action
RIFM fragrance ingredient safety assessment, Valencene, CAS Registry Number 4630-07-3
Parenteral microemulsions: an overview
Temperate pasture: management for grazing and conservation
Evaluation of a new coprocessed compound based on lactose and maize starch for tablet formulation
Thermal expansion and cracking of three confined water-saturated igneous rocks to 800C
Revision of the genera of the tribe Stigmoderini (Coleoptera: Buprestidae) a discussion of phylogenetic relationships
Anal tuberculosis. Report of a case
Gastric tuberculosis in the past and present
Adaptive responses of the cardiovascular system to prolonged spaceflight conditions: assessment with Holter monitoring

Changes in mitochondrial Ca2+ detected with Rhod-2 in single frog and mouse skeletal muscle fibres during and after repeated tetanic contractions


Changes in mitochondrial Ca2+ detected with Rhod-2 in single frog and mouse skeletal muscle fibres during and after repeated tetanic contractions



Journal of Muscle Research and Cell Motility 22(3): 265-275



ISSN/ISBN: 0142-4319

PMID: 11763199

DOI: 10.1023/a:1012227009544

The present study investigated mitochondrial Ca2+ uptake and release in intact living skeletal muscle fibres subjected to bouts of repetitive activity. Confocal microscopy was used in conjunction with the Ca2+-sensitive dye Rhod-2 to monitor changes in mitochondrial Ca2+ in single Xenopus or mouse muscle fibres. A marked increase in the mitochondrial Ca2+ occurred in Xenopus fibres after 10 tetani applied at 4 s intervals. The mitochondrial Ca2+ continued to increase with increasing number of tetani. After the end of tetanic stimulation, mitochondrial Ca2+ declined to 50% of the maximal increase within 10 min and thereafter took up to 60 min to return to its original value. Depolarization of the mitochondria with FCCP greatly attenuated the rise in the mitochondrial Ca2+ evoked by repetitive tetanic stimulation. In addition, FCCP slowed the rate of decay of the tetanic Ca2+ transient which in turn led to an elevation of resting cytosolic Ca2+. Accumulation of Ca2+ in the mitochondria was accompanied by a modest mitochondrial depolarization. In contrast to the situation in Xenopus fibres, mitochondria in mouse toe muscle fibres did not show any change in the mitochondrial Ca2+ during repetitive stimulation and FCCP had no effect on the rate of decay of the tetanic Ca2+ transient. It is concluded that in Xenopus fibres, mitochondria play a role in the regulation of cytosolic Ca2+ and contribute to the relaxation of tetanic Ca2+ transients. In contrast to their important role in Xenopus fibres, mitochondria in mouse fast-twitch skeletal fibres play little role in Ca2+ homeostasis.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 045477437

Download citation: RISBibTeXText

Related references

Mitochondrial Ca2+ in mouse soleus single muscle fibres in response to repeated tetanic contractions. Advances in Experimental Medicine and Biology 538: 557-62; Discussion 562, 2003

Mitochondrial and myoplasmic (Ca2+) in single fibres from mouse limb muscles during repeated tetanic contractions. Journal of Physiology (Cambridge) 551(1): 179-190, 2003

Mitochondrial and myoplasmic [Ca2+] in single fibres from mouse limb muscles during repeated tetanic contractions. Journal of Physiology 551(Pt 1): 179-190, 2003

Intracellular and mitochondrial Ca2+ in mouse intact soleus skeletal muscle fibres during repeated intermittent tetanic stimulation. Journal of Physiology (Cambridge) 543P: 102P, 2002

Oxygen uptake of frog skeletal muscle fibres following tetanic contractions at 18 degrees C. Journal of Physiology 346: 365-377, 1984

Effects of hydrostatic pressure on isometric twitch and tetanic contractions of single intact frog muscle fibres. Journal of Physiology (Cambridge) 475P: 34P, 1994

Energy liberation and chemical change in frog skeletal muscle during single isometric tetanic contractions. Journal of General Physiology 65(1): 1-21, 1975

Relaxation, (Ca-2+)-i and (Mg-2+)-i during prolonged tetanic stimulation of intact, single fibres from mouse skeletal muscle. Journal of Physiology (Cambridge) 480(1): 31-43, 1994

Relaxation, [Ca2+]i and [Mg2+]i during prolonged tetanic stimulation of intact, single fibres from mouse skeletal muscle. Journal of Physiology 480: 31-43, 1994

Intersarcomere dynamics during fixed-end tetanic contractions of frog muscle fibres. Journal of Physiology 293: 365-378, 1979

Effect of release of hydrostatic pressure during isometric contractions of single frog skeletal muscle fibres. Journal of Physiology (Cambridge) 476P: 14P, 1994

Intracellular calcium and force in single mouse muscle fibres following repeated contractions with stretch. Journal of Physiology 488: 25-36, 1995

The effects of verapamil on tetanic contractions of frog's skeletal muscle. Comparative Biochemistry and Physiology. Pharmacology Toxicology and Endocrinology 107(3): 321-329, 1994

The variation of characteristics of twitch and tetanic contractions with sarcomere length in isolated muscle fibres of the frog. Archivio di Fisiologia 71(1-4): 279-302, 1979

Enhancement of mechanical performance by stretch during tetanic contractions of vertebrate skeletal muscle fibres. The Journal of Physiology 281(1): 139-155, 1978