+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Characterization of a coronavirus isolated from a diarrheic foal



Characterization of a coronavirus isolated from a diarrheic foal



Journal of Clinical Microbiology 38(12): 4523-4526



A coronavirus was isolated from feces of a diarrheic foal and serially propagated in human rectal adenocarcinoma (HRT-18) cells. Antigenic and genomic characterizations of the virus (isolate NC99) were based on serological comparison with other avian and mammalian coronaviruses and sequence analysis of the nucleocapsid (N) protein gene. Indirect fluorescent-antibody assay procedures and virus neutralization assays demonstrated a close antigenic relationship with bovine coronavirus (BCV) and porcine hemagglutinating encephalomyelitis virus (mammalian group 2 coronaviruses). Using previously described BCV primers, the N protein gene of isolate NC99 was amplified by a reverse transcriptase PCR (RT-PCR) procedure. The RT-PCR product was cloned into pUC19 and sequenced; the complete N protein of NC99 (446 amino acids) was then compared with published N protein sequences of other avian and mammalian coronaviruses. A high degree of identity (89.0 to 90.1%) was observed between the N protein sequence of NC99 and published sequences of BCV (Mebus and F15 strains) and human coronavirus (strain OC43); only limited identity (<25%) was observed with group 1 and group 3 coronaviruses. Based on these findings, the virus has been tentatively identified as equine coronavirus (ECV). ECV NC99 was determined to have close antigenic and/or genetic relationships with mammalian group 2 coronaviruses, thus identifying it as a member of this coronavirus antigenic group.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 045488764

Download citation: RISBibTeXText

PMID: 11101590


Related references

An enteric coronavirus in a 3-day-old diarrheic foal. Arquivos do Instituto Biologico Sao Paulo 73(1): 101-103, 2006

Characterization of virulence factors and phylogenetic group determination of Escherichia coli isolated from diarrheic and non-diarrheic calves from Brazil. Folia Microbiologica 62(2): 139-144, 2017

Longitudinal study of Salmonella spp., diarrheagenic Escherichia coli, Rotavirus, and Coronavirus isolated from healthy and diarrheic calves in a Brazilian dairy herd. Tropical Animal Health and Production 47(1): 3, 2015

Molecular characterization of canine parvovirus and canine enteric coronavirus in diarrheic dogs on the island of St. Kitts: First report from the Caribbean region. Virus Research 240: 154-160, 2017

Subtypes Distribution and Frequency of Blastocystis sp. Isolated from Diarrheic and Non-diarrheic Patients. Iranian Journal of Parasitology 12(1): 63-68, 2017

Inter- and intra-subtype variation of Blastocystis subtypes isolated from diarrheic and non-diarrheic patients in Iran. Infection Genetics and Evolution 50: 77-82, 2017

Characterization of a parvovirus isolated from the diarrheic feces of a pig. Nihon Juigaku Zasshi. Japanese Journal of Veterinary Science 51(2): 337-344, 1989

Prevalence of adhesin and toxin genes in E. coli strains isolated from diarrheic and non-diarrheic pigs from smallholder herds in northern and eastern Uganda. Bmc Microbiology 16(1): 178, 2016

Serogroups, virulence genes and antibiotic resistance in Shiga toxin-producing Escherichia coli isolated from diarrheic and non-diarrheic pediatric patients in Iran. Gut Pathogens 5(1): 39, 2013

Comparison of electrocardiographic parameters and serum electrolytes and microelements between single infection of rotavirus and coronavirus and concurrent infection ofCryptosporidium parvumwith rotavirus and coronavirus in diarrheic dairy calves. Comparative Clinical Pathology 21(3): 241-244, 2012

Characterization and significance of aeromonas spp isolated from diarrheic stools in nigeria. Journal of Tropical Medicine & Hygiene 92(2): 97-101, 1989

Characterization of eae+ Escherichia coli isolated from healthy and diarrheic calves. Veterinary Microbiology 66(4): 251-263, 1999

Prevalence, toxin gene profile, antibiotic resistance, and molecular characterization of Clostridium perfringens from diarrheic and non-diarrheic dogs in Korea. Journal of Veterinary Science 19(3): 368-374, 2018

Detection and molecular characterization of Cryptosporidium spp. isolated from diarrheic children in Switzerland. Pediatric Infectious Disease Journal 23(4): 359-361, 2004

Molecular characterization of bovine Cryptosporidium isolated from diarrheic calves in the Sudan. Parasitology Research 116(11): 2971-2979, 2017