+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Characterization of coronavirus DI RNA packaging

Characterization of coronavirus DI RNA packaging

Advances in Experimental Medicine and Biology 440: 347-353

Studies of defective interfering (DI) RNAs of mouse hepatitis virus (MHV), suggest that a 69 nt-long packaging signal, which is located about 20 kb from the 5'-end of the 31 kb-long MHV genomic RNA, is necessary and sufficient for MHV genomic RNA packaging into MHV particles. We demonstrated that use of a low pH culture medium combined with subsequent ultrafiltration increased MHV infectivity about 60 times over MHV preparations grown in neutral medium. Using this virus concentration procedure, we successfully prepared DI particle-rich MHV preparations. Characterization of virus samples released from the cells infected with DI particle-rich MHV revealed that infectious MHV genomic RNA was not required for packaging of DI RNAs. These data suggested that interaction of the DI packaging signal with an unidentified region(s) of helper virus genomic RNA is unlikely, and therefore unlikely to facilitate the packaging of MHV DI RNA into the MHV virion. Rather, both DI RNA and MHV genomic RNA probably use the packaging signal for RNA packaging.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 045490578

Download citation: RISBibTeXText

PMID: 9782302

Related references

Identification and characterization of a coronavirus packaging signal. Journal of Virology 66(6): 3522-3530, 1992

Molecular characterization of transmissible gastroenteritis coronavirus defective interfering genomes: packaging and heterogeneity. Virology 217(2): 495-507, 1996

Cooperation of an RNA packaging signal and a viral envelope protein in coronavirus RNA packaging. Journal of Virology 75(19): 9059-9067, 2001

Murine coronavirus packaging signal confers packaging to nonviral RNA. Journal of Virology 71(1): 824-827, 1997

Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia. Journal of Virology 79(2): 884-895, 2004

Isolation and characterization of a novel Betacoronavirus subgroup A coronavirus, rabbit coronavirus HKU14, from domestic rabbits. Journal of Virology 86(10): 5481-5496, 2012

Identification and characterization of a novel alpaca respiratory coronavirus most closely related to the human coronavirus 229E. Viruses 4(12): 3689-3700, 2012

Isolation and Characterization of a Novel Bat Coronavirus Closely Related to the Direct Progenitor of Severe Acute Respiratory Syndrome Coronavirus. Journal of Virology 90(6): 3253-3256, 2015

Characterization of the nonstructural and spike proteins of the human respiratory coronavirus OC43: comparison with bovine enteric coronavirus. Advances in Experimental Medicine and Biology 342: 61-67, 1993

Characterization of the Lipidomic Profile of Human Coronavirus-Infected Cells: Implications for Lipid Metabolism Remodeling upon Coronavirus Replication. Viruses 11(1):, 2019

Genomic characterization of SARS coronavirus: a novel member of coronavirus. Yi Chuan Xue Bao 30(6): 501-508, 2003

Isolation and Characterization of Dromedary Camel Coronavirus UAE-HKU23 from Dromedaries of the Middle East: Minimal Serological Cross-Reactivity between MERS Coronavirus and Dromedary Camel Coronavirus UAE-HKU23. International Journal of Molecular Sciences 17(5):, 2016

Coronavirus genome packaging. 2007

Molecular characterization and pathogenesis of transmissible gastroenteritis coronavirus (TGEV) and porcine respiratory coronavirus (PRCV) field isolates co-circulating in a swine herd. Archives of Virology 145(6): 1133-1147, 2000

Identification of a bovine coronavirus packaging signal. Journal of Virology 74(1): 580-583, 2000