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Characterization of necrotoxigenic Escherichia coli (NTEC) strains isolated from healthy calves in Poland

Osek, J.

Journal of Veterinary Medicine. B Infectious Diseases and Veterinary Public Health 48(9): 641-646

2001


ISSN/ISBN: 0931-1793
PMID: 11765799
DOI: 10.1046/j.1439-0450.2001.00481.x
Accession: 045492162

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Faecal samples from 132 healthy, 4-8-week-old calves from four different farms were examined for necrotoxigenic Escherichia coli (NTEC) producing the cytotoxic necrotizing factors type 1 (CNF1) and type 2 (CNF2). CNF2 genes were detected by polymerase chain reaction in 24 (6.1%) of the 396 E. coli strains tested; these strains were found in 18 (13.6%) calves used in the study. None of the 396 E. coli isolates examined possessed the gene encoding CNF1. Overall, 28.8% of E. coli examined expressed the F17 fimbrial antigen. A strong association between CNF2 toxin and F17 fimbriae was found (62.5% of CNF2-positive strains were F17-positive). Moreover, six out of 24 NTEC strains had the Stx1 or the Stx2 shiga toxin genes, and three additional isolates possessed the eae genetic marker of the intimin protein.

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