+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Clonal expansion and decreased occurrence of peripheral blood gamma delta T cells of the V delta 2J delta 3 lineage in multiple sclerosis patients



Clonal expansion and decreased occurrence of peripheral blood gamma delta T cells of the V delta 2J delta 3 lineage in multiple sclerosis patients



International Immunology 9(7): 1031-1041



gamma delta T cells are implicated in autoimmune diseases but their precise function remains unclear. In multiple sclerosis (MS) brain tissue, gamma delta T cells co-localize with heat shock protein (hsp)65+ oligodendrocytes and are oligoclonally restricted in the V delta 2J delta 3 lineage. To investigate the homing properties and the degree of heterogeneity of V delta 2J delta 3+ gamma delta T cells in MS, peripheral blood lymphocytes (PBL) from 34 MS patients, 42 controls (14 autoimmune control patients, 28 healthy volunteers), and 11 lymphatic tissues of MS patients and controls were studied by RT-PCR and sequencing. V delta 2J delta 3 TCR rearrangement was detected in 27 out of 28 healthy controls, and was significantly less frequent in MS patients (24 out of 34) and autoimmune control patients (seven out of 14). It was present only in five out of 11 tissue specimens, none of them from MS patients. Direct sequencing and cloning/automated sequencing of the V delta 2J delta 3 PCR products indicated heterogeneity in healthy controls and oligoclonality in MS patients, but also in autoimmune control patients. Differences between MS patients and healthy controls were more accentuated in exacerbating hospitalized patients than in clinically stable outpatients participating in a clinical trial. Only one V delta 2J delta 3 sequence of a total of 85 different sequences obtained was shared between two MS patients. Taken together, evidence for clonal expansion of V delta 2J delta 3+ gamma delta T cells was found in PBL of MS patients. This T cell subset, previously shown to be present in 100% of chronic-active MS plaques, might home to the CNS in MS, resulting in its under-representation in the blood.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 045547508

Download citation: RISBibTeXText

PMID: 9237112

DOI: 10.1093/intimm/9.7.1031


Related references

Clonal expansion of gamma/delta T cells in peripheral blood of multiple sclerosis patients as evidence by restricted TCR-delta chain genes repertoire. 9TH INTERNATIONAL CONGRESS OF IMMUNOLOGY [Author] The 9th International Congress of Immunology : 256, 1995

Peripheral expansion of V delta 1-J delta 1/J delta 2+ gamma delta T cells and large granular lymphocytes in a patient with Wiskott-Aldrich syndrome. Acta Paediatrica Japonica 37(3): 394-398, 1995

Oligoclonal expansion of V-delta-1+gamma/delta cells in multiple tissues of systemic sclerosis patients. Clinical Research 42(2): 142A, 1994

Expansion of V-delta-1/V-gamma-8 expressing and V-gamma dual expressing gamma-delta T cells in peripheral blood of patients with inflammatory bowel disease. 9TH INTERNATIONAL CONGRESS OF IMMUNOLOGY [Author] The 9th International Congress of Immunology : 252, 1995

Monooligoclonal expansion of gamma/delta T cells in multiple sclerosis as evidenced by restricted pattern of TCR delta chain gene rearrangement on RNA level. Immunology Letters 73(2-3): 166, 2000

Oligoclonal expansion of V delta 1+ gamma/delta T-cells in systemic sclerosis patients. Annals of the new York Academy of Sciences 756: 382-391, 1995

Expansion of selected V delta 1+ gamma delta T cells in systemic sclerosis patients. Journal of Immunology 153(2): 881-891, 1994

The presence of an endogenous murine leukemia virus sequence correlates with the peripheral expansion of gamma delta T cells bearing the BALB invariant delta (BID) T cell receptor delta. Journal of Experimental Medicine 178(5): 1819-1824, 1993

Patterns of chemokine receptor expression on peripheral blood gamma delta T lymphocytes: strong expression of CCR5 is a selective feature of V delta 2/V gamma 9 gamma delta T cells. Journal of Immunology 168(10): 4920-4929, 2002

Zoledronic Acid-Mediated Ex Vivo Expansion and Function of Human Umbilical Cord Blood Gamma Delta T Cells Is Distinct from Human Peripheral Blood Gamma Delta T Cells. Biology of Blood and Marrow Transplantation 21(2): S241-S242, 2015

Gamma delta T lymphocytes and their V gamma 9 and V delta 2 receptor expression in peripheral blood mononuclear cells of active tuberculosis patients before and after treatment. Kurume Medical Journal 47(1): 5, 2000

CDR3-independent gamma delta V delta 1+ T cell expansion in the peripheral blood of HIV-infected persons. Journal of Immunology 154(3): 1418-1431, 1995

Peripheral blood gamma delta T cells in multiple sclerosis. Rinsho Shinkeigaku 33(11): 1131-1134, 1993

Clonal expansions of V delta 1+ and V delta 2+ cells increase with age and limit the repertoire of human gamma delta T cells. European Journal of Immunology 24(8): 1914-1918, 1994

Diversity of V delta-J delta gene rearrangement in peripheral blood lymphocytes and intrathecal IgG synthesis in multiple sclerosis. Folia Neuropathologica 37(2): 113-116, 1999