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Cloning, functional expression and tissue distribution of human cDNA for the alpha 1C-adrenergic receptor

Hirasawa, A.; Horie, K.; Tanaka, T.; Takagaki, K.; Murai, M.; Yano, J.; Tsujimoto, G.

Biochemical and Biophysical Research Communications 195(2): 902-909

1993


ISSN/ISBN: 0006-291X
PMID: 8396931
DOI: 10.1006/bbrc.1993.2130
Accession: 045551573

We have cloned human alpha 1C-adrenergic receptor from human prostate cDNA library. The deduced amino acid sequence of the clone (P2C4) encodes a protein of 466 amino acids that showed strong sequence homology to the previously cloned bovine alpha 1C-adrenergic receptor. The radioligand binding properties of P2C4 expressed in COS-7 cells were very similar to those of bovine alpha 1C-adrenergic receptor. With reverse-transcription polymerase chain reaction assay, we observed alpha 1C-adrenergic receptor transcripts in heart, brain, liver and prostate, but not in kidney, lung, adrenal, aorta and pituitary. The data show that the clone P2C4 encodes a human alpha 1C-adrenergic receptor cDNA, and the receptor subtype is expressed not widely but localized in several human tissues.

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