Section 46
Chapter 45,552

Cloning, functional expression and tissue distribution of rabbit alpha 1d-adrenoceptor

Suzuki, F.; Miyamoto, S.; Takita, M.; Oshita, M.; Watanabe, Y.; Kakizuka, A.; Narumiya, S.; Taniguchi, T.; Muramatsu, I.

Biochimica et Biophysica Acta 1323(1): 6-11


ISSN/ISBN: 0006-3002
PMID: 9030207
DOI: 10.1016/s0005-2736(96)00229-5
Accession: 045551574

We have cloned a cDNA encoding rabbit alpha 1d-adrenoceptor from the rabbit liver cDNA library. The deduced amino-acid sequence of this clone encodes a protein of 576 amino acids that shows strong sequence homology to previously cloned human, rat and mouse alpha 1d-adrenoceptors. The pharmacological radioligand binding properties of this clone expressed in COS-7 cells were similar to those of rat alpha 1d-adrenoceptors. Competitive RT/PCR assays revealed wide tissue distribution of the alpha 1d-adrenoceptor mRNA in rabbit, especially abundant in vas deferens, aorta, prostate and cerebral cortex.

PDF emailed within 0-6 h: $19.90