+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

A Fluorescence-Based Assay for Identification of Bacterial Topoisomerase I Poisons

A Fluorescence-Based Assay for Identification of Bacterial Topoisomerase I Poisons

Methods in Molecular Biology 1703: 259-268

Bacterial Topoisomerase I is a potential target for the identification of novel topoisomerase poison inhibitors that could provide leads for a new class of antibacterial compounds. Here we describe in detail a fluorescence-based cleavage assay that is successfully used in HTS for the discovery of bacterial topoisomerase Ι poisons.

(PDF emailed within 0-6 h: $19.90)

Accession: 045571409

Download citation: RISBibTeXText

PMID: 29177747

DOI: 10.1007/978-1-4939-7459-7_18

Related references

A solid phase assay for topoisomerase I interfacial poisons and catalytic inhibitors. Analytical Biochemistry 421(2): 607-616, 2012

A high-throughput fluorescence anisotropy-based assay for human topoisomerase II β-catalyzed ATP-dependent supercoiled DNA relaxation. Analytical Biochemistry 448: 23-29, 2014

A high-throughput-compatible, fluorescence anisotropy-based assay for ATP-dependent supercoiled DNA relaxation by human topoisomerase IIα. Biochemical Pharmacology 85(9): 1269-1277, 2013

Identification of novel cytotoxic noncamptothecin topoisomerase I poisons. Proceedings of the American Association for Cancer Research Annual Meeting 44: 347, 2003

A combination fluorescence assay and Folin-Ciocalteau phenol reagent assay for the detection of paralytic shellfish poisons. Toxicon 23(3): 375-381, 1985

Discovery of acetyl-coenzyme A carboxylase 2 inhibitors: comparison of a fluorescence intensity-based phosphate assay and a fluorescence polarization-based ADP Assay for high-throughput screening. Assay and Drug Development Technologies 5(2): 225-235, 2007

Genome-Wide Identification and Characterization of Novel Factors Conferring Resistance to Topoisomerase II Poisons in Cancer. Cancer Research 75(19): 4176-4187, 2016

Identification of early gene expression changes in primary cultured neurons treated with topoisomerase I poisons. Biochemical and Biophysical Research Communications 479(2): 319-324, 2016

A camptothecin-resistant DNA topoisomerase I mutant exhibits altered sensitivities to other DNA topoisomerase poisons. Journal of Biological Chemistry 270(11): 6141-6148, 1995

A mutation in yeast topoisomerase II that confers hypersensitivity to multiple classes of topoisomerase II poisons. Journal of Biological Chemistry 275(11): 7980-7987, 2000

Genetic elements suppressing human topoisomerase ii and inducing resistance to topoisomerase ii poisons. Proceedings of the American Association for Cancer Research Annual Meeting 33: 453, 1992

Modulation of topoisomerase I and IIalpha levels in tumour cells upon exposure to topoisomerase poisons. British Journal of Cancer 80(SUPPL 2): 50, 1999

Coordinating the two protomer active sites of human topoisomerase IIalpha: nicks as topoisomerase II poisons. Biochemistry 48(7): 1439-1441, 2009

Natural products as topoisomerase II poisons: effects of thymoquinone on DNA cleavage mediated by human topoisomerase IIα. Chemical Research in Toxicology 27(5): 787-793, 2015

Inhibition of NF-kappaB and proteasome activity in tumors: can we improve the therapeutic potential of topoisomerase I and topoisomerase II poisons. Current Pharmaceutical Design 8(22): 1945-1958, 2002