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A Fluorescence-Based Assay for Identification of Bacterial Topoisomerase I Poisons



A Fluorescence-Based Assay for Identification of Bacterial Topoisomerase I Poisons



Methods in Molecular Biology 1703: 259-268



Bacterial Topoisomerase I is a potential target for the identification of novel topoisomerase poison inhibitors that could provide leads for a new class of antibacterial compounds. Here we describe in detail a fluorescence-based cleavage assay that is successfully used in HTS for the discovery of bacterial topoisomerase Ι poisons.

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Accession: 045571409

Download citation: RISBibTeXText

PMID: 29177747

DOI: 10.1007/978-1-4939-7459-7_18


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