Comparison between enzyme-linked immunosorbent assay, indirect fluorescent antibody and rapid conglutination tests in detecting antibodies against Babesia bovis
Araújo, F.R.; Madruga, C.R.; Leal, C.R.; Schenk, M.A.; Kessler, R.H.; Marques, A.P.; Lemaire, D.C.
Veterinary Parasitology 74(2-4): 101-108
ISSN/ISBN: 0304-4017 PMID: 9561698 DOI: 10.1016/s0304-4017(97)00171-4
The performance of an enzyme-linked immunosorbent assay (ELISA), an indirect fluorescent antibody test (IFAT) and a rapid conglutination test (RCT) for the detection of antibodies against Babesia bovis, was evaluated with 462 cattle sera from Bahia State; Brazil. The results showed a concordance of 96.6% between the ELISA and IFAT, 90.5% between the ELISA and RCT, and 91.8% between the IFAT and RCT. Although the prevalence rates determined by ELISA (97.2%) and IFAT (96.8%) were higher than that indicated by the RCT (92.9%), performances of the three serological tests were very similar and characterized the region studied as enzootically stable to B. bovis.