+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Coronavirus MHV-A59 causes upregulation of interferon-beta RNA in primary glial cell cultures



Coronavirus MHV-A59 causes upregulation of interferon-beta RNA in primary glial cell cultures



Advances in Experimental Medicine and Biology 440: 451-454



Infection of mice with coronavirus mouse hepatitis virus strain MHV-A59 causes focal acute encephalitis, hepatitis and chronic demyelinating disease. To investigate host interferon (IFN) response to viral infection within the brain, RNA was extracted from A59-or MHV-2- infected and mock-infected primary astrocyte cultures from newborn mice, RT-PCR amplified RNA with primers specific for the various IFNs, transferred to nylon membranes and hybridized with IFN specific digoxigenin-labeled probes. Infection of primary astrocyte cultures from newborn mice with either A59 or MHV-2 caused upregulation of IFN-beta RNA, but not IFN-gamma or IFN-alpha. Thus, brain astrocytes are capable of producing a local IFN-beta response upon infection with MHV. The response of the other IFNs following MHV infection may be derived from inflammatory cells.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 045652143

Download citation: RISBibTeXText

PMID: 9782314


Related references

Expression of DSD-1-PG in primary neural and glial-derived cell line cultures, upregulation by TGF-beta, and implications for cell-substrate interactions of the glial cell line Oli-neu. Glia 23(2): 99, 1998

Coronavirus mouse hepatitis virus (MHV)-A59 causes a persistent, productive infection in primary glial cell cultures. Microbial Pathogenesis 3(2): 79-86, 1987

Growth pattern of various jhm coronavirus isolates in primary rat glial cell cultures correlates with differing neurotropism in vivo. Virus Research 9(2-3): 133-144, 1988

Interferon-beta inhibits the expression of metalloproteinases in rat glial cell cultures: implications for multiple sclerosis pathogenesis and treatment. Multiple Sclerosis 10(3): 290-297, 2004

Effect of hydrocortisone and thyroxine on aryl sulfatases and beta galactosidase of primary cell cultures of neuronal and glial types. Journal of Neurochemistry 30(6): 1614-1616, 1978

Glial cell functions studied with primary cultures and cell lines of glial origin. Naunyn-Schmiedeberg's Archives of Pharmacology 321(Suppl.): R11, 1982

Alterations in the glial fibrillary acidic protein content of primary astrocyte cultures for evaluation of glial cell toxicity. Toxicology In Vitro. 8(3): 351-359, 1994

Characteristics of the beta 1-and beta 2-adrenergic-sensitive adenylate cyclases in glial cell primary cultures and their comparison with beta 2-adrenergic-sensitive adenylate cyclase of meningeal cells. Brain Research 213(1): 151-161, 1981

Differential upregulation of HLA class-I molecules on neuronal and glial cells by virus-infection correlates with differential induction of interferon-beta. 9TH INTERNATIONAL CONGRESS OF IMMUNOLOGY [Author] The 9th International Congress of Immunology : 358, 1995

Beta-Amyloid regulates gene expression of glial trophic substance S100 beta in C6 glioma and primary astrocyte cultures. Brain Research. Molecular Brain Research 34(1): 118-126, 1995

Interferon regulatory factor 3 is required for viral induction of beta interferon in primary cardiac myocyte cultures. Journal of Virology 73(12): 10208-10213, 1999

Heparin-binding epidermal growth factor, in combination with basic fibroblast growth factor, promotes glial cell process formation in primary glial cell cultures. Society for Neuroscience Abstracts 23(1-2): 2439, 1997

Interferon-beta downregulates interferon-gamma-induced class II MHC molecule expression and morphological changes in primary cultures of human brain microvessel endothelial cells. Journal of Neuroimmunology 60(1-2): 63-73, 1995